Synthetic nanodiscs offer a stable and biologically relevant environment that closely mimics cell membranes and enables full-length transmembrane human 5-HT-5A protein to be purified and analysed in vitro.
Ausdruckssystem
HEK293 cells
Natur
Synthetic
Spezies
Human
Proteinlänge
Full length protein.
Molekulargewicht
Full length human 5-HT-5A protein has a MW of 40.3 kDa.
Konjugat
Unconjugated
Produktform
Lyophilized
Konzentration
Reconstitution dependent.
Formulierung
Lyophilized from nanodisc solubilization buffer (20 mM Tris-HCl, 150 mM NaCl, pH 8.0). Normally 5% - 8% trehalose is added as protectants before lyophilization.
Lagerung
Shipped at 4°C. Lyophilized: Store at -20°C to -80°C. Reconstituted: Aliquot and store at -80°C. Product is stable for one year. Avoid freeze/thaw cycles.
Figure 1: Synthetic Nanodisc Human 5-HT-5A Protein (A325081)
Diagram showing how synthetic nanodiscs containing full-length multi-pass transmembrane proteins in a phospholipid bilayer are generated from native cell membranes.
