Anti-Syndecan-1 Antibody [DM46] - BSA and Azide free
Beschreibung
Recombinant rabbit monoclonal [DM46] antibody to Syndecan-1.
Anwendungen
ELISA, Flow Cytometry
Verdünnungen
ELISA: 1:5,000-10,000, Flow Cytometry: 1:100
Reaktivität
Human
Wirt
Rabbit
Klonalität
Monoclonal
Klon
DM46
Isotyp
IgG
Konjugat
Unconjugated
Reinigung
Affinity chromatography.
Konzentration
Reconstitution dependent.
Produktform
Lyophilized
Rekonstitution
Reconstitute with distilled sterile water.
Formulierung
Lyophilized from sterile Phosphate Buffered Saline, pH 7.4. Normally 5%–8% Trehalose is added as a protectant before lyophilization.
Lagerung
Shipped at 4°C. Lyophilized: Store at -20°C to -80°C. Reconstituted: Aliquot and store at -80°C. Product is stable for one year. Avoid freeze/thaw cycles.
Allgemeine Hinweise
Prior to reconstitution, centrifuge the vial at 5,000g for 3-5 minutes at room temperature. Reconstitute with appropriate volume of distilled sterile water to bring product to 1mg/ml concentration. After addition of distilled sterile water, mix by gentle tapping. Note: It is not recommended to vortex or vigorously pipette the sample.
Expi 293 cell line transfected with irrelevant protein (A) and human CD138 (B) were surface stained with Anti-Syndecan-1 Antibody [DM46] - Azide free (A318649) at 1 µg/ml followed by Anti-Rabbit IgG Antibody (Alexa 488).
Flow cytometry data of serially titrated Anti-Syndecan-1 Antibody [DM46] - Azide free (A318649) on H929 cells. The Y-axis represents the mean fluorescence intensity (MFI) while the X-axis represents the concentration of IgG used.
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