This SYK (phospho Tyr323) Cell Based ELISA Kit allows for the detection of SYK (phospho Tyr323) and the effects that certain stimulation conditions have on SYK (phospho Tyr323) expression in different cell lines. Qualitative determination of SYK (phospho Tyr323) concentration is achieved by an indirect ELISA format. In essence, SYK (phospho Tyr323) is captured by Anti-SYK (phospho Tyr323) Antibodies while Dye 1-conjugated and Dye 2-conjugated secondary antibodies bind the Fc region of the primary antibody. Through this binding, the dye conjugated to the secondary antibody can emit light at a certain wavelength given proper excitation, hence allowing for a fluorometric detection method. A monoclonal antibody specific for human GAPDH is included to serve as an internal positive control in normalizing the target RFU values. Anti-SYK Antibody is also included for normalization purposes. The RFU values obtained for the non-phosphorylated target can be used to normalize the RFU value for the phosphorylated target.
Figure 4: Western Blot - SYK (phospho Tyr323) Cell Based ELISA Kit (FLUO-CBP1219)
The mouse monoclonal antibody to GAPDH is used as a positive control in SYK (phospho Tyr323) Cell Based ELISA Kit. Anti-GAPDH Antibody was tested for specificity by western blot with tissue lysates from human, mouse, and rat.
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