Rabbit polyclonal antibody to STAT5B (Phospho-Ser731)
Spezifität
The antibody detects endogenous levels of STAT5B only when phosphorylated at serine 731.
Anwendungen
WB, IHC
Reaktivität
Human, Mouse, Rat
Immunogen
Peptide sequence around phosphorylation site of serine 731 (A-P-S(p)-P-A) derived from Human STAT5B.
Wirt
Rabbit
Klonalität
Polyclonal
Konjugat
Unconjugated
Reinigung
Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.
Konzentration
1 mg/ml
Formulierung
Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Lagerung
Store at -20˚C
Synonyme
Signal transducer and activator of transcription 5B, STA5B_HUMAN, STAT5, Transcription factor STAT5B
Western blot analysis of extracts from RAW264.7 cells, treated with EGF (200ng/ml, 30mins), using STAT5B (phospho-Ser731) antibody #12152. The lane on the right is treated with the synthesized peptide.
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using STAT5B (phospho-Ser731) antibody #12152. The picture on the right is treated with the synthesized peptide.
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