Figure 1: Western Blot - Anti-STAT4 Antibody (A15468)
Western blot analysis of extracts of NCI-H460 cells, using Anti-STAT4 Antibody (A15468) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 90s.
Immunohistochemistry analysis of paraffin-embedded rat spleen using Anti-STAT4 Antibody (A15468) at a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
Immunohistochemistry analysis of paraffin-embedded human rectum using Anti-STAT4 Antibody (A15468) at a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
Immunohistochemistry analysis of paraffin-embedded mouse spleen using Anti-STAT4 Antibody (A15468) at a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
![Western Blot - Anti-STAT4 Antibody [ARC1071] (A306876) - Antibodies.com](https://cdn.antibodies.com/image/catalog/306/A306876_1.jpg?profile=product_alternative)
