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Anti-Staphylococcus aureus Aptamer [SA20] (Biotin) (A320965)

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Name
Anti-Staphylococcus aureus Aptamer [SA20] (Biotin)
Description
Synthetic DNA aptamer to Staphylococcus aureus [SA20] (Biotin).
Specificity
Streptococcus and S.epidermidis were used as counter targets.
Conjugate

Biotin

Aptamer Resuspension
Resuspend to 100µM with 10mM Tris-HCl, pH 7.5, and 0.1mM EDTA.
Apatamer Refolding Conditions
Denatured at 100°C for 5 min and then cooled (immediately) on ice for 10 min.
Apatmer Binding Conditions
0.1mg/ml Salmon Sperm DNA, 0.1mg/ml tRNA, 1% Bovine Serum Albumin, Phosphate Buffered Saline, and 0.05% (v/v) Tween 20. Binding Temperature: 37°C.
Sequence
5'-dGpdCpdApdApdTpdGpdGpdTpdApdCpdGpdGpdTpdApdCpdTpdTpdCpdCpdGpdCpdGpdCpdCpdCpdTpdCpdTpdCpdApdCpdGpdTpdGpdGpdCpdApdCpdTpdCpdApdGpdApdGpdTpdGpdCpdCpdGpdGpdApdApdGpdTpdTpdCpdTpdGpdCpdGpdTpdTpdApdTpdCpdApdApdApdApdGpdTpdGpdCpdApdCpdGpdCpdTpdApdCpdTpdTpdTpdGpdCpdTpdApdAp-3'
Aptamer Length
88 nt
Aptamer Molecular Weight (Da)
27,053.55 Da
Aptamer Affinity
Affinity (Kd) = 70.86 nM
Extinction Coefficient
822700 M-1cm-1
Purification
HPLC
Product Form
Lyophilized
Concentration
Reconstitution dependent.
Formulation
Lyophilized from nuclease-free water (no residual salts).
Storage
Shipped at 4°C. Lyophilized: Store at -20°C. Reconstituted: Store short term at 4°C (i.e. use within days) or aliquot and store at -20°C (medium term: i.e. use within weeks) or -80°C long term. Avoid freeze/thaw cycles.
Product Notes
Anti-Staphylococcus aureus Aptamer [SA20] (Biotin) (A320965) is a replica of the aptamer described in PMID: 23223587.
General Notes
Aptamers have not been validated by Antibodies.com and they need to be tested/evaluated under identical conditions to which they were reported in the literature including, but not limited to, temperature and matrix/buffer conditions. All aptamers are manufactured on request and have a lead time of 30-45 business days.
Disclaimer
This product is for research use only. It is not intended for diagnostic or therapeutic use.
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