Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol and 0.05% Proclin 300.
Storage
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Synonyms
EBI3-associated protein of 60 kDa, EBIAP, ORCA, OSIL, p60, Phosphotyrosine-independent ligand for the Lck SH2 domain of 62 kDa, Sequestosome-1, Ubiquitin-binding protein p62
Western Blot - Anti-SQSTM1 / p62 Antibody (A309479)
Western blot analysis of extracts of various cell lines, using Anti-SQSTM1 / p62 Antibody (A309479) at 1:4,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 3s.
Western Blot - Anti-SQSTM1 / p62 Antibody (A309479)
Western blot analysis of extracts from wild type(WT) and SQSTM1/p62 knockout (KO) 293T cells, using Anti-SQSTM1 / p62 Antibody (A309479) at 1:4,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 3s.
Immunohistochemistry analysis of paraffin-embedded human lung adenocarcinoma using Anti-SQSTM1 / p62 Antibody (A309479) at a dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
Immunohistochemistry analysis of paraffin-embedded human extranodal NK-T cell lymphoma using Anti-SQSTM1 / p62 Antibody (A309479) at a dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
Immunohistochemistry analysis of paraffin-embedded mouse kidney using Anti-SQSTM1 / p62 Antibody (A309479) at a dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
Immunohistochemistry analysis of paraffin-embedded rat spleen using Anti-SQSTM1 / p62 Antibody (A309479) at a dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
Immunofluorescence analysis of NIH/3T3 cells using Anti-SQSTM1 / p62 Antibody (A309479) at a dilution of 1:100. DAPI was used to stain the cell nuclei (blue).
Immunofluorescence analysis of C6 cells using Anti-SQSTM1 / p62 Antibody (A309479) at a dilution of 1:100. DAPI was used to stain the cell nuclei (blue).
Immunofluorescence analysis of HeLa cells using Anti-SQSTM1 / p62 Antibody (A309479) at a dilution of 1:200 (40x lens). DAPI was used to stain the cell nuclei (blue).
Immunofluorescence analysis of HepG2 cells using Anti-SQSTM1 / p62 Antibody (A309479) at a dilution of 1:200 (40x lens). DAPI was used to stain the cell nuclei (blue).
Immunofluorescence analysis of NIH/3T3 cells using Anti-SQSTM1 / p62 Antibody (A309479) at a dilution of 1:200 (40x lens). DAPI was used to stain the cell nuclei (blue).
Immunofluorescence analysis of PC-12 cells using Anti-SQSTM1 / p62 Antibody (A309479) at a dilution of 1:200 (40x lens). DAPI was used to stain the cell nuclei (blue).
Immunofluorescence analysis of A-549 cells using Anti-SQSTM1 / p62 Antibody (A309479) at a dilution of 1:100 (40x lens). DAPI was used to stain the cell nuclei (blue).
Immunofluorescence analysis of NIH/3T3 cells using Anti-SQSTM1 / p62 Antibody (A309479) at a dilution of 1:100 (40x lens). DAPI was used to stain the cell nuclei (blue).
Immunofluorescence analysis of U2OS cells using Anti-SQSTM1 / p62 Antibody (A309479) at a dilution of 1:100 (40x lens). DAPI was used to stain the cell nuclei (blue).
Western Blot - Anti-SQSTM1 / p62 Antibody (A309479)
Immunoprecipitation analysis of 200µg extracts of HeLa cells using 3µg of Anti-SQSTM1 / p62 Antibody (A309479). This Western blot was performed on the immunoprecipitate using Anti-SQSTM1 / p62 Antibody (A309479) at a dilution of 1:1000.
Western Blot - Anti-SQSTM1 / p62 Antibody (A309479)
Immunoprecipitation analysis of 600µg extracts of mouse liver using 3µg of Anti-SQSTM1 / p62 Antibody (A309479). This Western blot was performed on the immunoprecipitate using Anti-SQSTM1 / p62 Antibody (A309479) at a dilution of 1:1000.
