Western blot analysis of extracts of MCF7 cells, using Anti-SOX2 Antibody (A12651) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 60s.
Western blot analysis of extracts of various cell lines, using Anti-SOX2 Antibody (A12651) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 180s.
Immunohistochemistry analysis of paraffin-embedded mouse embryos using Anti-SOX2 Antibody (A12651) at a dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
Immunofluorescence analysis of F9 cells using Anti-SOX2 Antibody (A12651) at a dilution of 1:50 (40x lens). DAPI was used to stain the cell nuclei (blue).
Immunoprecipitation analysis of 200µg extracts of C6 cells using 3µg of Anti-SOX2 Antibody (A12651). This Western blot was performed on the immunoprecipitate using Anti-SOX2 Antibody (A12651) at a dilution of 1:500.
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