Anti-SOX10 Antikörper [PCRP-SOX10-1D8] (A253198)

Name: Anti-SOX10 Antibody [PCRP-SOX10-1D8] - BSA and Azide free
Brand: Antibodies.com
SKU: A253198
Price: 525.0 USD
Availability: InStock

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$525

Monoklonal [PCRP-SOX10-1D8] Maus Antikörper gegen SOX10 für IHC-P.

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Alternative Formats and ConjugatesAlternative Formats

Unconjugated

Versand

Versandkosten

$40

Vorlaufzeit

Lieferung in 4-6 Werktagen

Kontakt

Telefon

+1 (314) 370-6046

orders@antibodies.com

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Stichworte:

Monospezifischer Antikörper Neuroepithelial Marker Oligodendrocyte Marker Schwann Cell Marker

Produktdetails

Produktname

Anti-SOX10 Antibody [PCRP-SOX10-1D8] - BSA and Azide free

Beschreibung

Mouse monoclonal [PCRP-SOX10-1D8] antibody to SOX10.

Spezifität

The specificity of this monoclonal antibody to its intended target was validated by HuProt^TM Array, containing more than 19,000 full-length human proteins. Recognizes a protein of ~55kDa, identified as SOX10. This MAb is highly specific and does not cross-react with other members of the SOX-family. SOX genes comprise a family of genes that are related to the mammalian sex-determining gene SRY. These genes similarly contain sequences that encode for the HMG-box domain, which is responsible for the sequence-specific DNA-binding activity. SOX10 is a sensitive marker of melanoma, including conventional, spindled, and desmoplastic subtypes. Expressed by metastatic melanomas and nodal capsular nevus in sentinel lymph nodes, but not by other lymph node components such as dendritic cells, which usually express S100 protein. Often used melanoma markers, such as anti-HMB45 and anti-MelanA, are poorly expressed in desmoplastic melanomas while SOX10 is moderately-to-strongly expressed in desmoplastic melanomas. SOX-10 is considered as a very reliable marker for recognizing residual desmoplastic melanomas. In normal tissues, it is expressed in Schwann cells, melanocytes mammary glands. SOX-10 expression is also observed in mast cells.

Anwendungen

IHC-P

Verdünnungen

IHC-P: 1-2 µg/ml

Reaktivität

Human

Kreuzreaktivität

This antibody is predicted to cross react with Mouse and Rat.

Immunogen

Recombinant fragment, around amino acids 90-179, of human SOX10 protein. The exact sequence is proprietary.

Wirt

Mouse

Klonalität

Monoclonal

Klon

PCRP-SOX10-1D8

Isotyp

IgG2b

Konjugat

Unconjugated

Reinigung

Protein A/G chromatography.

Konzentration

1 mg/ml

Molekulargewicht

49-58 kDa

Produktform

Liquid

Formulierung

Supplied in 10mM Phosphate Buffered Saline; without Sodium Azide and carrier free.

Lagerung

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Allgemeine Hinweise

This monoclonal antibody is also available in a different formulation with BSA and Sodium Azide - Anti-SOX10 Antibody [PCRP-SOX10-1D8] (A250018).

Synonyme

DOM, Dominant megacolon mouse human homolog of, MGC15649, PCWH, SOX 10, SOX10_HUMAN, SRY (sex determining region Y) box 10, SRY box 10, SRY box containing gene 10, SRY related HMG box gene 10, Transcription factor SOX 10, Transcription factor SOX-10, WS2E, WS4, WS4C

Isotyp-Kontrollantikörper

Kompatible Sekundärantikörper

Haftungsausschluss

Dieses Produkt ist nur für Forschungszwecke bestimmt. Es ist nicht für diagnostische oder therapeutische Zwecke bestimmt.

Produktbilder (4)

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Figure 1: Immunofluorescence - Anti-SOX10 Antibody [PCRP-SOX10-1D8] - BSA and Azide free (A253198)

Immunofluorescent analysis of PFA fixed HeLa cells stained with Anti-SOX10 Antibody [PCRP-SOX10-1D8] followed by Goat Anti-Mouse IgG (CF® 488) (Green). Counterstain is Phalloidin (Red).

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Figure 2: Flow Cytometry - Anti-SOX10 Antibody [PCRP-SOX10-1D8] - BSA and Azide free (A253198)

Flow cytometric analysis of PFA fixed HeLa cells using Anti-SOX10 Antibody [PCRP-SOX10-1D8] followed by Goat Anti-Mouse IgG (CF® 488) (Blue). Unstained cells (red).

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Figure 3: Anti-SOX10 Antibody [PCRP-SOX10-1D8] - BSA and Azide free (A253198)

Analysis of protein array containing more than 19,000 full-length human proteins using Anti-SOX10 Antibody [PCRP-SOX10-1D8]. Z-Score and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody (MAb) (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProt^TM array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If targets on HuProt^TM are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-score. S-score therefore represents the relative target specificity of a MAb to its intended target; a MAb is considered to be specific to its intended target, if the MAb has an S-score of at least 2.5. For example, if a MAb binds to protein X with a Z-score of 43 and to protein Y with a Z-score of 14, then the S-score for the binding of that MAb to protein X is equal to 29.

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Figure 4: SDS-PAGE - Anti-SOX10 Antibody [PCRP-SOX10-1D8] - BSA and Azide free (A253198)

SDS-PAGE analysis of Anti-SOX10 Antibody [PCRP-SOX10-1D8] under non-reduced and reduced conditions; showing intact IgG and intact heavy and light chains, respectively. SDS-PAGE analysis confirms the integrity and purity of the antibody.