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SNAP25 Cell Based Kit Elisa (A102979)

Western Blot - SNAP25 Cell Based ELISA Kit (CB5644) - Antibodies.com Protocol Diagram - SNAP25 Cell Based ELISA Kit (CB5644) - Antibodies.com Protocol Summary - SNAP25 Cell Based ELISA Kit (CB5644) - Antibodies.com Western Blot - Control Antibody - SNAP25 Cell Based ELISA Kit (CB5644) - Antibodies.com
$550
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$40
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Lieferung in 5-8 Werktagen

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Produktname
SNAP25 Cell Based ELISA Kit
Assay-Typ
Cell-based (qualitative)
Assay-Prinzip
This SNAP25 Cell Based ELISA Kit allows for the detection of SNAP25 and the effects that certain stimulation conditions have on SNAP25 expression in different cell lines. Qualitative determination of SNAP25 concentration is achieved by an indirect ELISA format. In essence, the SNAP25 is captured by Anti-SNAP25 Antibodies which in turn are detected by HRP-conjugated secondary antibodies. Through this binding, the HRP enzyme (conjugated to the secondary antibody) can catalyze a colorimetric reaction upon substrate addition. Due to the qualitative nature of this SNAP25 Cell Based ELISA Kit, multiple normalization methods are described: 1) Anti-GAPDH Antibody is included to serve as an internal positive control in normalizing the target absorbance values. 2) Following the colorimetric measurement of HRP activity via substrate addition, the Crystal Violet whole-cell staining method is used to determine cell density. After staining, the results can be analyzed by normalizing the absorbance values to cell amounts, by which the plating difference can be adjusted.
Plattform
Microplate
Nachweismethode
Colorimetric
Probentyp
Adherent cells and suspension cells.
Detektionsbereich
> 5000 Cells
Testdauer
4 hours 30 minutes
Reaktivität
Human, Mouse, Rat
Precision
Intra-assay CV: < 8%, Inter-assay CV: < 10%
Lagerung
Store at + 4°C. Stable for 6 months.
Synonyms
bA416N4.2, Bdr, CMS18, dJ1068F16.2, FLJ23079, HGNC:11132, MGC105414, MGC139754, Resistance to inhibitors of cholinesterase 4 homolog, RIC 4, RIC4, SEC 9, SEC9, SNAP, SNAP 25, SNAP-25, SNAP-25B, SNP 25, SNP25, SNP25_HUMAN, sp, SUP, Super protein, Synaptosomal associated 25 kDa protein, Synaptosomal associated protein, Synaptosomal associated protein 25, Synaptosomal associated protein 25kDa, Synaptosomal-associated 25 kDa protein, Synaptosomal-associated protein 25, Synaptosomal-associated protein, 25-KD
Haftungsausschluss
Dieses Produkt ist nur für Forschungszwecke bestimmt. Es ist nicht für diagnostische oder therapeutische Zwecke bestimmt.
Western Blot - SNAP25 Cell Based ELISA Kit (CB5644) - Antibodies.comBild Vergrößern
Figure 1: Western Blot - SNAP25 Cell Based ELISA Kit (CB5644)
Western blot analysis of extracts from Raw264.7 cells, treated with EGF 200ng/ml 30', using Anti-SNAP25 Antibody.
Protocol Diagram - SNAP25 Cell Based ELISA Kit (CB5644) - Antibodies.comBild Vergrößern
Figure 2: SNAP25 Cell Based ELISA Kit (CB5644)
A diagram showing the protocol for SNAP25 Cell Based ELISA Kit.
Protocol Summary - SNAP25 Cell Based ELISA Kit (CB5644) - Antibodies.comBild Vergrößern
Figure 3: SNAP25 Cell Based ELISA Kit (CB5644)
A summary of the protocol for SNAP25 Cell Based ELISA Kit.
Western Blot - Control Antibody - SNAP25 Cell Based ELISA Kit (CB5644) - Antibodies.comBild Vergrößern
Figure 4: Western Blot - SNAP25 Cell Based ELISA Kit (CB5644)
The mouse monoclonal antibody to GAPDH is used as a positive control inSNAP25 Cell Based ELISA Kit. Anti-GAPDH Antibody was tested for specificity by western blot with tissue lysates from human, mouse, and rat.
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