Anti-SMURF 2 Antikörper [ARC1897] (A308190)

Name: Anti-SMURF 2 Antibody [ARC1897]
Brand: Antibodies.com
SKU: A308190
Price: 470.0 USD
Availability: InStock

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$470

Monoklonal [ARC1897] Kaninchen Antikörper gegen SMURF 2 für WB, IHC, ICC/IF und IP.

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Versand

Versandkosten

$40

Vorlaufzeit

Lieferung in 5-8 Werktagen

Kontakt

Telefon

+1 (314) 370-6046

orders@antibodies.com

Garantien

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Produktdetails

Produktname

Anti-SMURF 2 Antibody [ARC1897]

Beschreibung

Rabbit monoclonal [ARC1897] antibody to SMURF 2.

Anwendungen

WB, IHC, ICC/IF, IP

Verdünnungen

WB: 1:500-1:2,000, IHC: 1:50-1:200, ICC/IF: 1:50-1:200, IP: 1:500-1:1,000

Reaktivität

Human, Mouse

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 201-290 of human SMURF2 (Q9HAU4).

Sequenz

PLSCFVDENTPISGTNGATCGQSSDPRLAERRVRSQRHRNYMSRTHLHTPPDLPEGYEQRTTQQGQVYFLHTQTGVSTWHDPRVPRDLSN

Wirt

Rabbit

Klonalität

Monoclonal

Klon

ARC1897

Isotyp

IgG

Konjugat

Unconjugated

Reinigung

Affinity purification.

Molekulargewicht

80 kDa

Produktform

Liquid

Formulierung

Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol, 0.05% BSA, and 0.02% Sodium Azide.

Lagerung

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Synonyme

E3 ubiquitin-protein ligase SMURF2, EC 6.3.2., hSMURF2, MGC138150, Smad specific E3 ubiquitin ligase 2, SMAD specific E3 ubiquitin protein ligase 2, SMAD ubiquitination regulatory factor 2, SMAD-specific E3 ubiquitin-protein ligase 2, SMUF2_HUMAN, Smurf2, Ubiquitin protein ligase SMURF2

Isotyp-Kontrollantikörper

Kompatible Sekundärantikörper

Haftungsausschluss

Dieses Produkt ist nur für Forschungszwecke bestimmt. Es ist nicht für diagnostische oder therapeutische Zwecke bestimmt.

Produktbilder (4)

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Figure 1: Western Blot - Anti-SMURF 2 Antibody [ARC1897] (A308190)

Western blot analysis of extracts of various cell lines, using Anti-SMURF 2 Antibody [ARC1897] (A308190) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Enhanced Kit (RM00021). Exposure time: 3min.

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Figure 2: Immunohistochemistry - Anti-SMURF 2 Antibody [ARC1897] (A308190)

Immunohistochemistry analysis of paraffin-embedded human colon tissue using Anti-SMURF 2 Antibody [ARC1897] (A308190) at a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

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Figure 3: Immunofluorescence - Anti-SMURF 2 Antibody [ARC1897] (A308190)

Immunofluorescence analysis of NIH-3T3 cells using Anti-SMURF 2 Antibody [ARC1897] (A308190) at a dilution of 1:100 (40x lens). DAPI was used to stain the cell nuclei (blue).

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Figure 4: Western Blot - Anti-SMURF 2 Antibody [ARC1897] (A308190)

Immunoprecipitation analysis of 300µg extracts of mouse lung using 3µg of Anti-SMURF 2 Antibody [ARC1897] (A308190). This Western blot was performed on the immunoprecipitate using Anti-SMURF 2 Antibody [ARC1897] (A308190) at a dilution of 1:1000.