Western blot analysis of extracts of various cell lines, using Anti-SMARCA6 Antibody (A15003) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 90s.
Western blot analysis of extracts from normal (control) and Lsh/HELLS knockout (KO) HeLa cells, using Anti-SMARCA6 Antibody (A15003) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 5s.
Immunoprecipitation analysis of 200µg extracts of 293T cells using 1µg of Anti-SMARCA6 Antibody (A15003). This Western blot was performed on the immunoprecipitate using Anti-SMARCA6 Antibody (A15003) at a dilution of 1:1000.
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