Anti-SMAD5 (phospho Ser463 + Ser465) Antikörper (A308813)

Name: Anti-SMAD5 (phospho Ser463 + Ser465) Antibody
Brand: Antibodies.com
SKU: A308813
Price: 435.0 USD
Availability: InStock

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$435

Polyklonal Kaninchen Antikörper gegen SMAD5 (phospho Ser463 + Ser465) für WB und IHC.

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Versand

Versandkosten

$40

Vorlaufzeit

Lieferung in 5-8 Werktagen

Kontakt

Telefon

+1 (314) 370-6046

orders@antibodies.com

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Produktdetails

Produktname

Anti-SMAD5 (phospho Ser463 + Ser465) Antibody

Beschreibung

Rabbit polyclonal antibody to SMAD5 (phospho Ser463 + Ser465).

Anwendungen

WB, IHC

Verdünnungen

WB: 1:500-1:1,000, IHC: 1:50-1:200

Reaktivität

Human, Mouse, Rat

Immunogen

A synthetic phosphorylated peptide around S463 & S465 of human Phospho-Smad5-S463/S465 (NP_005894.3).

Sequenz

SSVS

Wirt

Rabbit

Klonalität

Polyclonal

Isotyp

IgG

Konjugat

Unconjugated

Reinigung

Affinity purification.

Produktform

Liquid

Formulierung

Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol and 0.05% Proclin 300.

Lagerung

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Synonyme

DKFZp781C1895, DKFZp781O1323, Dwfc, hSmad5, JV5 1, JV5-1, MAD homolog 5, MAD, mothers against decapentaplegic homolog 5, MADH 5, MADH5, Mothers against decapentaplegic homolog 5, mothers against decapentaplegic, drosophila, homolog of, 5, Mothers against DPP homolog 5, MusMLP, SMA and MAD related protein 5, SMAD 5, SMAD family member 5, SMAD, mothers against DPP homolog 5, SMAD5_HUMAN

Isotyp-Kontrollantikörper

Kompatible Sekundärantikörper

Haftungsausschluss

Dieses Produkt ist nur für Forschungszwecke bestimmt. Es ist nicht für diagnostische oder therapeutische Zwecke bestimmt.

Produktbilder (4)

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Figure 1: Western Blot - Anti-SMAD5 (phospho Ser463 + Ser465) Antibody (A308813)

Western blot analysis of extracts of NIH/3T3, using Anti-SMAD5 (phospho Ser463 + Ser465) Antibody (A308813) at 1:600 dilution. NIH/3T3 cells were treated by BMP4 (50 ng/ml) at 37°C for 30 minutes after serum-starvation overnight. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 90s.

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Figure 2: Immunohistochemistry - Anti-SMAD5 (phospho Ser463 + Ser465) Antibody (A308813)

Immunohistochemistry analysis of paraffin-embedded human kidney using Anti-SMAD5 (phospho Ser463 + Ser465) Antibody (A308813) at a dilution of 1:50 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

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Figure 3: Immunohistochemistry - Anti-SMAD5 (phospho Ser463 + Ser465) Antibody (A308813)

Immunohistochemistry analysis of paraffin-embedded mouse kidney using Anti-SMAD5 (phospho Ser463 + Ser465) Antibody (A308813) at a dilution of 1:50 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

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Figure 4: Immunohistochemistry - Anti-SMAD5 (phospho Ser463 + Ser465) Antibody (A308813)

Immunohistochemistry analysis of paraffin-embedded rat kidney using Anti-SMAD5 (phospho Ser463 + Ser465) Antibody (A308813) at a dilution of 1:50 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.