Anti-SMAD5 Antikörper (A90354)

Name: Anti-SMAD5 Antibody
Brand: Antibodies.com
SKU: A90354
Price: 335.0 USD
Availability: InStock

Bild Vergrößern

$335

Polyklonal Kaninchen Antikörper gegen SMAD5 für WB, IHC und ICC/IF.

100% Qualitätsgarantie

Preis-Match-Garantie

Versand

Versandkosten

$40

Vorlaufzeit

Lieferung in 5-8 Werktagen

Kontakt

Telefon

+1 (314) 370-6046

orders@antibodies.com

Garantien

100% Qualitätsgarantie

Preis-Match-Garantie

Produktdetails

Produktname

Anti-SMAD5 Antibody

Beschreibung

Rabbit polyclonal antibody to SMAD5.

Anwendungen

WB, IHC, ICC/IF

Verdünnungen

WB: 1:500-1:2,000, IHC: 1:50-1:200, ICC/IF: 1:50-1:200

Reaktivität

Human, Mouse, Rat

Immunogen

A synthetic peptide corresponding to a sequence within amino acids 200-300 of human Smad5 (NP_005894.3).

Sequenz

STYPNSPASSGPGSPFQLPADTPPPAYMPPDDQMGQDNSQPMDTSNNMIPQIMPSISSRDVQPVAYEEPKHWCSIVYYELNNRVGEAFHASSTSVLVDGFT

Wirt

Rabbit

Klonalität

Polyclonal

Isotyp

IgG

Konjugat

Unconjugated

Reinigung

Affinity purification.

Molekulargewicht

52 kDa

Produktform

Liquid

Formulierung

Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol and 0.01% Thiomersal.

Lagerung

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Synonyme

DKFZp781C1895, DKFZp781O1323, Dwfc, hSmad5, JV5 1, JV5-1, MAD homolog 5, MAD, mothers against decapentaplegic homolog 5, MADH 5, MADH5, Mothers against decapentaplegic homolog 5, mothers against decapentaplegic, drosophila, homolog of, 5, Mothers against DPP homolog 5, MusMLP, SMA and MAD related protein 5, SMAD 5, SMAD family member 5, SMAD, mothers against DPP homolog 5, SMAD5_HUMAN

Isotyp-Kontrollantikörper

Kompatible Sekundärantikörper

Haftungsausschluss

Dieses Produkt ist nur für Forschungszwecke bestimmt. Es ist nicht für diagnostische oder therapeutische Zwecke bestimmt.

Produktbilder (4)

Bild Vergrößern

Figure 1: Western Blot - Anti-SMAD5 Antibody (A90354)

Western blot analysis of extracts of various cell lines, using Anti-SMAD5 Antibody (A90354) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 30s.

Bild Vergrößern

Figure 2: Immunohistochemistry - Anti-SMAD5 Antibody (A90354)

Immunohistochemistry analysis of paraffin-embedded rat ovary using Anti-SMAD5 Antibody (A90354) at a dilution of 1:200 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

Bild Vergrößern

Figure 3: Immunohistochemistry - Anti-SMAD5 Antibody (A90354)

Immunohistochemistry analysis of paraffin-embedded human breast cancer tissue using Anti-SMAD5 Antibody (A90354) at a dilution of 1:200 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

Bild Vergrößern

Figure 4: Immunofluorescence - Anti-SMAD5 Antibody (A90354)

Immunofluorescence analysis of HeLa cells using Anti-SMAD5 Antibody (A90354) at a dilution of 1:100 (40x lens). DAPI was used to stain the cell nuclei (blue).