Anti-Smad3 (phospho Thr179) Antikörper (A11032)

Name: Anti-Smad3 (phospho Thr179) Antibody
Brand: Antibodies.com
SKU: A11032
Price: 435.0 USD
Availability: InStock

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$435

Polyklonal Kaninchen Antikörper gegen Smad3 (phospho Thr179) für WB und IHC.

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Versand

Versandkosten

$40

Vorlaufzeit

Lieferung in 5-8 Werktagen

Kontakt

Telefon

+1 (314) 370-6046

orders@antibodies.com

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Produktdetails

Produktname

Anti-Smad3 (phospho Thr179) Antibody

Beschreibung

Rabbit polyclonal antibody to Smad3 (phospho Thr179).

Anwendungen

WB, IHC

Verdünnungen

WB: 1:500-1:2,000, IHC: 1:50-1:200

Reaktivität

Human, Mouse, Rat

Immunogen

A synthetic phosphorylated peptide around T179 of human Smad3 (NP_005893.1).

Sequenz

PETPP

Wirt

Rabbit

Klonalität

Polyclonal

Isotyp

IgG

Konjugat

Unconjugated

Reinigung

Affinity purification.

Molekulargewicht

48 kDa

Produktform

Liquid

Formulierung

Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol and 0.01% Thiomersal.

Lagerung

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Synonyme

DKFZP586N0721, DKFZp686J10186, hMAD 3, hMAD-3, hSMAD3, HSPC193, HST17436, JV15 2, JV15-2, JV152, LDS1C, LDS3, MAD (mothers against decapentaplegic Drosophila) homolog 3, MAD homolog 3, Mad homolog JV15 2, Mad protein homolog, MAD, mothers against decapentaplegic homolog 3, Mad3, MADH 3, MADH3, MGC60396, Mothers against decapentaplegic homolog 3, Mothers against DPP homolog 3, SMA and MAD related protein 3, SMAD, SMAD 3, SMAD family member 3, SMAD, mothers against DPP homolog 3, SMAD3_HUMAN

Isotyp-Kontrollantikörper

Kompatible Sekundärantikörper

Haftungsausschluss

Dieses Produkt ist nur für Forschungszwecke bestimmt. Es ist nicht für diagnostische oder therapeutische Zwecke bestimmt.

Produktbilder (5)

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Figure 1: Western Blot - Anti-Smad3 (phospho Thr179) Antibody (A11032)

Western blot analysis of extracts of HeLa cell line, using Anti-Smad3 (phospho Thr179) Antibody (A11032). The lane on the right is treated with the antigen-specific peptide. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% BSA.

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Figure 2: Western Blot - Anti-Smad3 (phospho Thr179) Antibody (A11032)

Western blot analysis of extracts of PC-12 cells, using Anti-Smad3 (phospho Thr179) Antibody (A11032) at 1:1,000 dilution. PC-12 cells were treated by TNF-alpha (20 ng/ml) at 37°C for 30 minutes after serum-starvation overnight. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% BSA. Detection was with a ECL Basic Kit. Exposure time: 30s.

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Figure 3: Immunohistochemistry - Anti-Smad3 (phospho Thr179) Antibody (A11032)

Immunohistochemistry analysis of paraffin-embedded human colon tissue using Anti-Smad3 (phospho Thr179) Antibody (A11032) at a dilution of 1:200 (40x lens). Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

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Figure 4: Immunohistochemistry - Anti-Smad3 (phospho Thr179) Antibody (A11032)

Immunohistochemistry analysis of paraffin-embedded mouse kidney using Anti-Smad3 (phospho Thr179) Antibody (A11032) at a dilution of 1:200 (40x lens). Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.