Anti-Smad3 (phospho Ser423 + Ser425) Antikörper (A309475)

Name: Anti-Smad3 (phospho Ser423 + Ser425) Antibody
Brand: Antibodies.com
SKU: A309475
Price: 435.0 USD
Availability: InStock

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$435

Polyklonal Kaninchen Antikörper gegen Smad3 (phospho Ser423 + Ser425) für WB.

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Versand

Versandkosten

$40

Vorlaufzeit

Lieferung in 5-8 Werktagen

Kontakt

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+1 (314) 370-6046

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Produktdetails

Produktname

Anti-Smad3 (phospho Ser423 + Ser425) Antibody

Beschreibung

Rabbit polyclonal antibody to Smad3 (phospho Ser423 + Ser425).

Anwendungen

Verdünnungen

WB: 1:500-1:1,000

Reaktivität

Human, Mouse

Immunogen

A phospho synthetic peptide corresponding to residues surrounding S423/S425 of Human SMAD3.

Wirt

Rabbit

Klonalität

Polyclonal

Isotyp

IgG

Konjugat

Unconjugated

Reinigung

Affinity purification.

Molekulargewicht

55 kDa

Produktform

Liquid

Formulierung

Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol and 0.05% Proclin 300.

Lagerung

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Synonyme

DKFZP586N0721, DKFZp686J10186, hMAD 3, hMAD-3, hSMAD3, HSPC193, HST17436, JV15 2, JV15-2, JV152, LDS1C, LDS3, MAD (mothers against decapentaplegic Drosophila) homolog 3, MAD homolog 3, Mad homolog JV15 2, Mad protein homolog, MAD, mothers against decapentaplegic homolog 3, Mad3, MADH 3, MADH3, MGC60396, Mothers against decapentaplegic homolog 3, Mothers against DPP homolog 3, SMA and MAD related protein 3, SMAD, SMAD 3, SMAD family member 3, SMAD, mothers against DPP homolog 3, SMAD3_HUMAN

Isotyp-Kontrollantikörper

Kompatible Sekundärantikörper

Haftungsausschluss

Dieses Produkt ist nur für Forschungszwecke bestimmt. Es ist nicht für diagnostische oder therapeutische Zwecke bestimmt.

Produktbilder (2)

Bild Vergrößern

Figure 1: Western Blot - Anti-Smad3 (phospho Ser423 + Ser425) Antibody (A309475)

Western blot analysis of extracts of various cell lines, using Anti-Smad3 (phospho Ser423 + Ser425) Antibody (A309475) at 1:1,000 dilution. Hela cells were treated by TGF-beta (10 ng/ml) at 37°C for 30 minutes. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 90s.

Bild Vergrößern

Figure 2: Western Blot - Anti-Smad3 (phospho Ser423 + Ser425) Antibody (A309475)

Western blot analysis of extracts of various cell lines, using Anti-Smad3 (phospho Ser423 + Ser425) Antibody (A309475) at 1:1,000 dilution. C2C12 cells were treated by TGF-beta (10 ng/ml) at 37°C for 30 minutes. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 180s.