Anti-Smad3 Antikörper (A304887)

Name: Anti-Smad3 Antibody
Brand: Antibodies.com
SKU: A304887
Price: 500.0 USD
Availability: InStock

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$500

Polyklonal Kaninchen Antikörper gegen Smad3 für WB und IHC.

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Versand

Versandkosten

$40

Vorlaufzeit

Lieferung in 5-8 Werktagen

Kontakt

Telefon

+1 (314) 370-6046

orders@antibodies.com

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Produktdetails

Produktname

Anti-Smad3 Antibody

Beschreibung

Rabbit polyclonal antibody to Smad3.

Spezifität

This antibody detects additional bands due to post translational modifications.

Anwendungen

WB, IHC

Verdünnungen

WB: 1:1,000, IHC: 1:50

Reaktivität

Human

Immunogen

Synthetic peptide of Human SMAD3 (110-210aa), conjugated to Keyhole Limpet Haemocyanin (KLH)

Wirt

Rabbit

Klonalität

Polyclonal

Isotyp

IgG

Konjugat

Unconjugated

Reinigung

Peptide affinity purification.

Konzentration

1 mg/ml

Molekulargewicht

48 kDa

Produktform

Liquid

Formulierung

Supplied in Phosphate Buffered Saline, pH 7.4, with 50% Glycerol and 0.09% Sodium Azide.

Lagerung

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Synonyme

DKFZP586N0721, DKFZp686J10186, hMAD 3, hMAD-3, hSMAD3, HSPC193, HST17436, JV15 2, JV15-2, JV152, LDS1C, LDS3, MAD (mothers against decapentaplegic Drosophila) homolog 3, MAD homolog 3, Mad homolog JV15 2, Mad protein homolog, MAD, mothers against decapentaplegic homolog 3, Mad3, MADH 3, MADH3, MGC60396, Mothers against decapentaplegic homolog 3, Mothers against DPP homolog 3, SMA and MAD related protein 3, SMAD, SMAD 3, SMAD family member 3, SMAD, mothers against DPP homolog 3, SMAD3_HUMAN

Isotyp-Kontrollantikörper

Kompatible Sekundärantikörper

Haftungsausschluss

Dieses Produkt ist nur für Forschungszwecke bestimmt. Es ist nicht für diagnostische oder therapeutische Zwecke bestimmt.

Produktbilder (2)

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Figure 1: Western Blot - Anti-Smad3 Antibody (A304887)

Western blot analysis of human Cervical cancer cell line (HeLa) lysate showing detection of ~48.1 kDa SMAD3 protein using Anti-Smad3 Antibody (A304887) at 1:1,000 for 2 hours at room temperature. Lane 1: Molecular Weight Ladder (MW). Lane 2: Cervical Cancer cell line (HeLa) lysate. Load: 10 µg. Block: 5% Skim Milk in 1X TBST. The secondary antibody used was Goat Anti-Rabbit HRP:IgG at 1:3000 for 1 hour at room temperature. Color Development: ECL solution for 5 minutes at room temperature. Predicted/Observed Size: ~48.1 kDa. Other Band(s): due to post translational modifications.

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Figure 2: Immunohistochemistry - Anti-Smad3 Antibody (A304887)

Immunohistochemistry analysis of human colonic adenocarcinoma, fixed in formalin and paraffin-embedded. The Primary Antibody used was Anti-Smad3 Antibody (A304887) at 1:50 for 30 minutes at room temperature. Counterstain: Hematoxylin. Magnification: 20X. HRP-DAB Detection.