Anti-Smad2 (phospho Ser465 + Ser467) Antibody [ARC56317] (A308802)

Name: Anti-Smad2 (phospho Ser465 + Ser467) Antibody [ARC56317]
Brand: Antibodies.com
SKU: A308802
Price: 485.0 USD
Availability: InStock

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$485

Rabbit monoclonal [ARC56317] antibody to Smad2 (phospho Ser465 + Ser467) for WB.

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Shipping Information

Freight/Packing Charges: $40

Dispatched from St. Louis, MO.

Lead Time: 7-10 business days.

Specifications

Name

Anti-Smad2 (phospho Ser465 + Ser467) Antibody [ARC56317]

Description

Rabbit monoclonal [ARC56317] antibody to Smad2 (phospho Ser465 + Ser467).

Applications

Dilutions

WB: 1:1,000-1:5,000

Reactivity

Human, Mouse

Immunogen

A synthetic phosphorylated peptide around S465 & S467 of human Phospho-Smad2-S465/S467SMAD2 (NP_005892.1).

Sequence

SSMS

Host

Rabbit

Clonality

Monoclonal

Clone ID

ARC56317

Isotype

IgG

Conjugate

Unconjugated

Purification

Affinity purification.

Concentration

Lot Specific

Molecular Weight

52 kDa / 60 kDa

Product Form

Liquid

Formulation

Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol, 0.05% BSA, and 0.05% Proclin 300.

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze/thaw cycles.

Synonyms

hMAD-2, hSMAD2, JV18-1, MAD homolog 2, Mad-related protein 2, MADH2, MADR2, Mothers against decapentaplegic homolog 2, Mothers against DPP homolog 2, SMAD 2, SMAD family member 2

Isotype Controls

Suitable Secondaries

See all Anti-Rabbit IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation DataValidation Data (1)

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Western Blot - Anti-Smad2 (phospho Ser465 + Ser467) Antibody [ARC56317] (A308802)

Western blot analysis of extracts of various lysates, using Anti-Smad2 (phospho Ser465 + Ser467) Antibody [ARC56317] (A308802) at 1:2,000 dilution. HeLa and NIH/3T3 were treated by TGF-beta3 (H) (200 ng/ml) at 37°C for 30 minutes after serum-starvation overnight. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 30s.

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