Anti-NKCC1 Antikörper (A84750)

Name: Anti-NKCC1 Antibody
Brand: Antibodies.com
SKU: A84750
Price: 480.0 USD
Availability: InStock

Dieses Produkt ist identisch mit Abcam (ab99558). Entdecken Sie mehr für weniger.

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$480

Polyklonal Ziege Antikörper gegen NKCC1 für ELISA, IHC, IF und Flow Cytometry.

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Versand

Versandkosten

$40

Vorlaufzeit

Lieferung in 3-5 Werktagen

Kontakt

Telefon

+1 (314) 370-6046

orders@antibodies.com

Garantien

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Produktdetails

Produktname

Anti-NKCC1 Antibody

Beschreibung

Goat polyclonal antibody to NKCC1.

Anwendungen

ELISA, IHC, IF, Flow Cytometry

Verdünnungen

ELISA: 1:64,000, IHC: 3.75µg/ml, IF: 10µg/ml, Flow Cytometry: 10µg/ml

Reaktivität

Human

Immunogen

Synthetic peptide corresponding to the internal region of human NKCC1.

Sequenz

C-NALQHSIRLSGVEDH

Wirt

Goat

Klonalität

Polyclonal

Isotyp

IgG

Konjugat

Unconjugated

Reinigung

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Konzentration

500 µg/ml

Produktform

Liquid

Formulierung

Supplied in Tris Buffered Saline, pH 7.3, with 0.5% BSA and 0.02% Sodium Azide.

Lagerung

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze/thaw cycles.

Synonyme

Basolateral Na-K-Cl symporter, BSC, BSC2, Bumetanide-sensitive sodium-(potassium)-chloride cotransporter 1, mBSC2, MGC104233, PPP1R141, Protein phosphatase 1, regulatory subunit 141, S12A2_HUMAN, Slc12a2, sodium-potassium-chloride cotransporter 1, Solute carrier family 12 (sodium/potassium/chloride transporter), member 2, solute carrier family 12 (sodium/potassium/chloride transporters) member 2, Solute carrier family 12 member 2, sy-ns

Isotyp-Kontrollantikörper

Goat IgG (A121671)

Kompatible Sekundärantikörper

Haftungsausschluss

Dieses Produkt ist nur für Forschungszwecke bestimmt. Es ist nicht für diagnostische oder therapeutische Zwecke bestimmt.

Produktbilder (3)

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Figure 1: Immunofluorescence - Anti-NKCC1 Antibody (A84750)

NKCC1 expression in HeLa cells analyzed by immunofluorescence. Cells were permeabilized with 0.15% Triton. Staining was performed with Anti-NKCC1 Antibody (A84750) at 10µg/ml for 1 hour and Alexa Fluor 488 secondary antibody at 2µg/ml. Cytoplasmic/vesicle and membrane staining shown and nuclei were stained with DAPI (blue). Negative control: Goat IgG Isotype Control at 10µg/ml followed by Alexa Fluor 488 secondary antibody at 2µg/ml.

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Figure 2: Flow Cytometry - Anti-NKCC1 Antibody (A84750)

NKCC1 expression in HeLa cells (blue line) analyzed by flow cytometry. Cells were fixed in PFA and permeabilized with 0.5% Triton. Staining was performed with Anti-NKCC1 Antibody (A84750) at 10µg/ml for 1 hour and Alexa Fluor 488 secondary antibody at 1µg/ml. Negative Control: Goat IgG Isotype Control (black line) followed by Alexa Fluor 488 secondary antibody.

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Figure 3: Immunohistochemistry - Anti-NKCC1 Antibody (A84750)

NKCC1 expression in Human Colon analyzed by immunohistochemistry. Tissue was paraffin-embedded, and antigen retrieval was achieved by steaming in citrate buffer, pH 6. Staining was performed with Anti-NKCC1 Antibody (A84750) at 3.75µg/ml and revealed with alkaline phosphatase (AP).