SHP-2 (phospho Tyr542) Cell Based ELISA Kit (A103803)

SHP-2 (phospho Tyr542) Cell Based ELISA Kit

Cell-based (qualitative)

This SHP-2 (phospho Tyr542) Cell Based ELISA Kit allows for the detection of SHP-2 (phospho Tyr542) and the effects that certain stimulation conditions have on SHP-2 (phospho Tyr542) expression in different cell lines. Qualitative determination of SHP-2 (phospho Tyr542) concentration is achieved by an indirect ELISA format. In essence, SHP-2 (phospho Tyr542) is captured by Anti-SHP-2 (phospho Tyr542) Antibodies while Dye 1-conjugated and Dye 2-conjugated secondary antibodies bind the Fc region of the primary antibody. Through this binding, the dye conjugated to the secondary antibody can emit light at a certain wavelength given proper excitation, hence allowing for a fluorometric detection method. A monoclonal antibody specific for human GAPDH is included to serve as an internal positive control in normalizing the target RFU values. Anti-SHP-2 Antibody is also included for normalization purposes. The RFU values obtained for the non-phosphorylated target can be used to normalize the RFU value for the phosphorylated target.

Fluorometric

> 5000 Cells

Human, Mouse, Rat

Store at + 4°C. Stable for 6 months.

Protein-tyrosine phosphatase 1D, Protein-tyrosine phosphatase 2C, PTP-1D, PTP-2C, PTP2C, PTPN11, SH-PTP2, SH-PTP3, SHPTP2, Tyrosine-protein phosphatase non-receptor type 11