Figure 1: Western Blot - Anti-SH2D3A Antibody (A84648)
SH2D3A expression in Human Tonsil lysate analyzed by western blot. Cells were lysed in RIPA buffer and 35µg protein was run per lane. Primary antibody incubation was performed with Anti-SH2D3A Antibody (A84648) at 0.1µg/ml and detected by chemiluminescence.
SH2D3A expression in Human Colon analyzed by immunohistochemistry. Tissue was paraffin-embedded, and antigen retrieval was achieved by steaming in citrate buffer, pH 6. Staining was performed with Anti-SH2D3A Antibody (A84648) at 5µg/ml and revealed with alkaline phosphatase (AP).
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