SGLT1 expression in isolated Brush Border Membranes from Human Kidney with (B) and without (A) immunizing peptide blockage analyzed by western blot. Cells were lysed and 70µg protein was run per lane. Staining was performed with Anti-SGLT1 Antibody (A326294) at 1µg/ml and detected by chemiluminescence.
SGLT1 expression in Human Small Intestine analyzed by immunohistochemistry. Tissue was paraffin-embedded, and antigen retrieval was achieved by steaming in citrate buffer, pH 6. Staining was performed with Anti-SGLT1 Antibody (A326294) at 3.8µg/ml and revealed with alkaline phosphatase (AP).
SGLT1 expression in PFA-perfused cryosections of Human Kidney analyzed by immunocytochemistry. Staining was performed with Anti-SGLT1 Antibody (A326294) at 2.5µg/ml overnight. Microwaved antigen retrieval with citrate buffer pH 6, CY3-staining.
SGLT1 expression in cryosections of Human Kidney analyzed by immunocytochemistry. Staining was performed with Anti-SGLT1 Antibody (A326294) at 0.1µg/ml.
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