Figure 1: Western Blot - Anti-Serine / threonine-protein kinase 4 / MST-1 Antibody (A16052)
Western blot analysis of extracts of various cells, using Anti-Serine / threonine-protein kinase 4 / MST-1 Antibody (A16052) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 15s.
Figure 2: Western Blot - Anti-Serine / threonine-protein kinase 4 / MST-1 Antibody (A16052)
Western blot analysis of extracts from normal (control) and MST1/STK4 knockout (KO) 293T cells, using Anti-Serine / threonine-protein kinase 4 / MST-1 Antibody (A16052) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 1s.
Figure 3: Western Blot - Anti-Serine / threonine-protein kinase 4 / MST-1 Antibody (A16052)
Immunoprecipitation analysis of 100µg extracts of HepG2 cells using 3µg of Anti-Serine / threonine-protein kinase 4 / MST-1 Antibody (A16052). This Western blot was performed on the immunoprecipitate using Anti-Serine / threonine-protein kinase 4 / MST-1 Antibody (A16052) at a dilution of 1:1000.
