Figure 1: Western Blot - Anti-Selenophosphate synthetase 1 Antibody (A89735)
Western blot analysis of extracts from wild type(WT) and SEPHS1 knockout (KO) 293T(KO) cells, using Anti-Selenophosphate synthetase 1 Antibody (A89735) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 1s.
