Figure 1: Western Blot - Anti-SCRIBBLE Antibody (A121159)
SCRIBBLE expression in HeLa (A), HepG2 (B) and U2OS (C) cell lysate analyzed by western blot. Cells were lysed in RIPA buffer and 35µg protein was run per lane. Primary antibody incubation was performed with Anti-SCRIBBLE Antibody (A121159) at 2µg/ml and detected by chemiluminescence.
SCRIBBLE expression in MCF7 cells analyzed by immunofluorescence. Cells were permeabilized with 0.15% Triton. Staining was performed with Anti-SCRIBBLE Antibody (A121159) at 10µg/ml for 1 hour and Alexa Fluor 488 secondary antibody at 2µg/ml. Cell junction staining shown and nuclei were stained with DAPI (blue). Negative control: Goat IgG Isotype Control at 10µg/ml followed by Alexa Fluor 488 secondary antibody at 2µg/ml.
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