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Anti-SARS-CoV-2 Spike Glycoprotein S1 Antibody (A309131)

Western Blot - Anti-SARS-CoV-2 Spike Glycoprotein S1 Antibody (A309131) - Antibodies.com ELISA - Anti-SARS-CoV-2 Spike Glycoprotein S1 Antibody (A309131) - Antibodies.com Immunofluorescence - Anti-SARS-CoV-2 Spike Glycoprotein S1 Antibody (A309131) - Antibodies.com Western Blot - Anti-SARS-CoV-2 Spike Glycoprotein S1 Antibody (A309131) - Antibodies.com
$345
Product Datasheet

Rabbit polyclonal antibody to SARS-CoV-2 Spike Glycoprotein S1 for WB, ICC/IF, IP and Dot Blot.

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Shipping Information

$40
Dispatched from St. Louis, MO.
Lead Time: 7-10 business days.

  • Specifications

Name
Anti-SARS-CoV-2 Spike Glycoprotein S1 Antibody
Description
Rabbit polyclonal antibody to SARS-CoV-2 Spike Glycoprotein S1.
Applications
WB, ICC/IF, IP, Dot Blot
Dilutions
Dot Blot: 1:500-1:2,000, ELISA: 1:20,000-1:80,000, WB: 1:500-1:1,000, ICC/IF: 1:100-1:500, IP: 1:2,000-1:10,000
Reactivity
SARS-CoV-2
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 11-682 of coronavirus Spike S1 (YP_009724390.1).
Sequence
VSSQCVNLTTRTQLPPAYTNSFTRGVYYPDKVFRSSVLHSTQDLFLPFFSNVTWFHAIHVSGTNGTKRFDNPVLPFNDGVYFASTEKSNIIRGWIFGTTLDSKTQSLLIVNNATNVVIKVCEFQFCNDPFLGVYYHKNNKSWMESEFRVYSSANNCTFEYVSQPFLMDLEGKQGNFKNLREFVFKNIDGYFKIYSKHTPINLVRDLPQGFSALEPLVDLPIGINITRFQTLLALHRSYLTPGDSSSGWTAGAAAYYVGYLQPRTFLLKYNENGTITDAVDCALDPLSETKCTLKSFTVEKGIYQTSNFRVQPTESIVRFPNITNLCPFGEVFNATRFASVYAWNRKRISNCVADYSVLYNSASFSTFKCYGVSPTKLNDLCFTNVYADSFVIRGDEVRQIAPGQTGKIADYNYKLPDDFTGCVIAWNSNNLDSKVGGNYNYLYRLFRKSNLKPFERDISTEIYQAGSTPCNGVEGFNCYFPLQSYGFQPTNGVGYQPYRVVVLSFELLHAPATVCGPKKSTNLVKNKCVNFNFNGLTGTGVLTESNKKFLPFQQFGRDIADTTDAVRDPQTLEILDITPCSFGGVSVITPGTNTSNQVAVLYQDVNCTEVPVAIHADQLTPTWRVYSTGSNVFQTRAGCLIGAEHVNNSYECDIPIGAGICASYQTQTNSPR
Host
Rabbit
Clonality
Polyclonal
Isotype
IgG
Conjugate

Unconjugated

Purification
Affinity purification.
Concentration
Lot Specific
Molecular Weight
110 kDa
Product Form
Liquid
Formulation
Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol and 0.01% Thiomersal.
Storage
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze/thaw cycles.
Isotype Controls
Suitable Secondaries
See all Anti-Rabbit IgG Secondaries →
Disclaimer
This product is for research use only. It is not intended for diagnostic or therapeutic use.

  • Scientific Validation DataValidation Data (4)

Western Blot - Anti-SARS-CoV-2 Spike Glycoprotein S1 Antibody (A309131) - Antibodies.comEnlarge Image

Western Blot - Anti-SARS-CoV-2 Spike Glycoprotein S1 Antibody (A309131)

Western blot analysis of extracts of 293T cells, using Anti-SARS-CoV-2 Spike Glycoprotein S1 Antibody (A309131) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 1s.
ELISA - Anti-SARS-CoV-2 Spike Glycoprotein S1 Antibody (A309131) - Antibodies.comEnlarge Image

Anti-SARS-CoV-2 Spike Glycoprotein S1 Antibody (A309131)

Anti-SARS-CoV-2 Spike Glycoprotein S1 Antibody (A309131) can bind immobilized recombinant SARS-COV-2 Spike S1 protein at 1 µg/ml (100 µl/well) with a linear range of 0.78-50ng/ml.
Immunofluorescence - Anti-SARS-CoV-2 Spike Glycoprotein S1 Antibody (A309131) - Antibodies.comEnlarge Image

Immunofluorescence - Anti-SARS-CoV-2 Spike Glycoprotein S1 Antibody (A309131)

Immunofluorescence analysis of 293T cells transfected with SARS-CoV-2 Spike S1 fusion protein (top left) and untreated 293T cells (top right) using Anti-SARS-CoV-2 Spike Glycoprotein S1 Antibody (A309131) at a dilution of 1:400 (40x lens). DAPI was used to stain the cell nuclei (blue).
Western Blot - Anti-SARS-CoV-2 Spike Glycoprotein S1 Antibody (A309131) - Antibodies.comEnlarge Image

Western Blot - Anti-SARS-CoV-2 Spike Glycoprotein S1 Antibody (A309131)

Immunoprecipitation analysis of 300µg extracts of 293T cells using 3µg of Anti-SARS-CoV-2 Spike Glycoprotein S1 Antibody (A309131). This Western blot was performed on the immunoprecipitate using Anti-SARS-CoV-2 Spike Glycoprotein S1 Antibody (A309131) at a dilution of 1:10000.
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