Western blot analysis of extracts of various cell lines, using Anti-S100A4 Antibody (A1631) at 1:1,000 dilution. Secondary antibody: Goat Anti-Rabbit IgG (H+L) (HRP) (AS014) at 1:10,000 dilution. Lysates / proteins: 25µg per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s.
Western blot analysis of extracts from normal (control) and S100A4 knockout (KO) HeLa cells, using Anti-S100A4 Antibody (A1631) at 1:1,000 dilution. Secondary antibody: Goat Anti-Rabbit IgG (H+L) (HRP) (AS014) at 1:10,000 dilution. Lysates / proteins: 25µg per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Enhanced Kit (RM00021). Exposure time: 180s.
![Immunohistochemistry - Anti-S100A4 Antibody [S100A4/1481] (A249920) - Antibodies.com](https://cdn.antibodies.com/image/catalog/249/A249920_1.jpg?profile=product_alternative)
![Immunohistochemistry - Anti-S100A4 Antibody [S100A4/1481] - BSA and Azide free (A253100) - Antibodies.com](https://cdn.antibodies.com/image/catalog/253/A253100_1.jpg?profile=product_alternative)
![Western Blot - Anti-S100A4 Antibody [ARC0475] (A305282) - Antibodies.com](https://cdn.antibodies.com/image/catalog/305/A305282_1.jpg?profile=product_alternative)
![Immunohistochemistry - Anti-S100A4 Antibody [S100A4/1482] (A249921) - Antibodies.com](https://cdn.antibodies.com/image/catalog/249/A249921_1.jpg?profile=product_alternative)
![Immunohistochemistry - Anti-S100A4 Antibody [S100A4/1482] - BSA and Azide free (A253101) - Antibodies.com](https://cdn.antibodies.com/image/catalog/253/A253101_1.jpg?profile=product_alternative)
![Immunohistochemistry - Anti-S100A4 Antibody [rS100A4/1481] (A249923) - Antibodies.com](https://cdn.antibodies.com/image/catalog/249/A249923_1.jpg?profile=product_alternative)
![Immunohistochemistry - Anti-S100A4 Antibody [rS100A4/1481] - BSA and Azide free (A253103) - Antibodies.com](https://cdn.antibodies.com/image/catalog/253/A253103_1.jpg?profile=product_alternative)
![Immunohistochemistry - Anti-S100A4 Antibody [S100A4/2750R] (A249924) - Antibodies.com](https://cdn.antibodies.com/image/catalog/249/A249924_1.jpg?profile=product_alternative)
![Immunohistochemistry - Anti-S100A4 Antibody [S100A4/2750R] - BSA and Azide free (A253104) - Antibodies.com](https://cdn.antibodies.com/image/catalog/253/A253104_1.jpg?profile=product_alternative)
![Protein Array - Anti-S100A4 Antibody [CPTC-S100A4-3] - BSA and Azide free (A253101) - Antibodies.com](https://cdn.antibodies.com/image/catalog/253/A253102_1.jpg?profile=product_alternative)
![Protein Array - Anti-S100A4 Antibody [CPTC-S100A4-3] (A249921) - Antibodies.com](https://cdn.antibodies.com/image/catalog/249/A249922_1.jpg?profile=product_alternative)