Mouse monoclonal [PCRP-RXRB-2B6] antibody to RXRB.
Spezifität
Two families of retinoid receptors, RARs and RXRs, have been identified. Retinoic acid receptors (RARs) include RAR, are activated by 9-cis-RA, a stereo- and photo-isomer of all trans-RA that is expressed in vivo in both liver and kidney and may represent a widely used hormone. As is true for the RAR subfamily, the RXR receptors are closely related to each other both in their DNA-binding and ligand-binding domains and are encoded by separate genes at distinct chromosomal loci.
Immunofluorescent analysis of PFA fixed HeLa cells stained with Anti-RXRB Antibody [PCRP-RXRB-2B6] followed by Goat Anti-Mouse IgG (CF® 488) (Green). Counterstain is Phalloidin-CF® 640A (Red).
Immunofluorescent analysis of PFA fixed K562 cells stained with Anti-RXRB Antibody [PCRP-RXRB-2B6] followed by Goat Anti-Mouse IgG (CF® 488) (Green). Counterstain is Phalloidin-CF® 640A (Red).
Flow cytometric analysis of PFA fixed HeLa cells using Anti-RXRB Antibody [PCRP-RXRB-2B6] followed by Goat Anti-Mouse IgG (CF® 488) (Blue). Unstained cells (red).
Analysis of protein array containing more than 19,000 full-length human proteins using Anti-RXRB Antibody [PCRP-RXRB-2B6]. Z-Score and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody (MAb) (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProtTM array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If targets on HuProtTM are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-score. S-score therefore represents the relative target specificity of a MAb to its intended target; a MAb is considered to be specific to its intended target, if the MAb has an S-score of at least 2.5. For example, if a MAb binds to protein X with a Z-score of 43 and to protein Y with a Z-score of 14, then the S-score for the binding of that MAb to protein X is equal to 29.
SDS-PAGE analysis of Anti-RXRB Antibody [PCRP-RXRB-2B6] under non-reduced and reduced conditions; showing intact IgG and intact heavy and light chains, respectively. SDS-PAGE analysis confirms the integrity and purity of the antibody.