Western blot analysis of extracts of various cell lines, using Anti-RRM1 Antibody (A91462) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST.
Immunofluorescence analysis of HeLa cells using Anti-RRM1 Antibody (A91462) at a dilution of 1:100 (40x lens). DAPI was used to stain the cell nuclei (blue).
Immunoprecipitation analysis of 200µg extracts of HeLa cells using 1µg of Anti-RRM1 Antibody (A91462). This Western blot was performed on the immunoprecipitate using Anti-RRM1 Antibody (A91462) at a dilution of 1:500.
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