Figure 1: Western Blot - Anti-RPL8 Antibody (Biotin) (A83650)
Anti-RPL8 Antibody (A83650 | Biotin) (0.3µg/ml) staining of HepG2 lysate (35µg protein in RIPA buffer), exactly mirroring its parental non-biotinylated product. Primary incubation was 1 hour. Detected by chemiluminescence, using streptavidin-HRP and using NAP blocker as a substitute for skimmed milk.