Anti-RPA70 Antibody [ARC0773] (A307959)

Name: Anti-RPA70 Antibody [ARC0773]
Brand: Antibodies.com
SKU: A307959
Price: 485.0 USD
Availability: InStock

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$485

Rabbit monoclonal [ARC0773] antibody to RPA70 for WB, IP and ChIP.

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Shipping Information

Freight/Packing Charges: $40

Dispatched from St. Louis, MO.

Lead Time: 7-10 business days.

Specifications

Name

Anti-RPA70 Antibody [ARC0773]

Description

Rabbit monoclonal [ARC0773] antibody to RPA70.

Applications

WB, IP, ChIP

Dilutions

WB: 1:500-1:2,000, IP: 1:500-1:1,000, ChIP: 1:500-1:1,000

Reactivity

Human, Rat

Immunogen

A synthetic peptide corresponding to a sequence within amino acids 1-100 of human RPA70/RPA1 (P27694).

Sequence

MVGQLSEGAIAAIMQKGDTNIKPILQVINIRPITTGNSPPRYRLLMSDGLNTLSSFMLATQLNPLVEEEQLSSNCVCQIHRFIVNTLKDGRRVVILMELE

Host

Rabbit

Clonality

Monoclonal

Clone ID

ARC0773

Isotype

IgG

Conjugate

Unconjugated

Purification

Affinity purification.

Concentration

Lot Specific

Molecular Weight

70 kDa

Product Form

Liquid

Formulation

Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol, 0.05% BSA, and 0.02% Sodium Azide.

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze/thaw cycles.

Isotype Controls

Suitable Secondaries

See all Anti-Rabbit IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation DataValidation Data (3)

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Western Blot - Anti-RPA70 Antibody [ARC0773] (A307959)

Western blot analysis of extracts of various cell lines, using Anti-RPA70 Antibody [ARC0773] (A307959) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 30s.

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Western Blot - Anti-RPA70 Antibody [ARC0773] (A307959)

Immunoprecipitation analysis of 300µg extracts of HeLa cells using 3µg of Anti-RPA70 Antibody [ARC0773] (A307959). This Western blot was performed on the immunoprecipitate using Anti-RPA70 Antibody [ARC0773] (A307959) at a dilution of 1:1000.

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Chromatin Immunoprecipitation - Anti-RPA70 Antibody [ARC0773] (A307959)

Chromatin immunoprecipitation (ChIP) analysis of extracts of HeLa cells, using Anti-RPA70 Antibody [ARC0773] (A307959) and Rabbit IgG. The amount of immunoprecipitated DNA was checked by quantitative PCR. Histogram was constructed by the ratios of the immunoprecipitated DNA to the input.

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