Anti-ROR gamma Antikörper (A82850)

Name: Anti-ROR gamma Antibody
Brand: Antibodies.com
SKU: A82850
Price: 480.0 USD
Availability: InStock

Dieses Produkt ist identisch mit Abcam (ab113434). Entdecken Sie mehr für weniger.

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$480

Polyklonal Ziege Antikörper gegen ROR gamma für ELISA, WB, IF und Flow Cytometry.

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Versand

Versandkosten

$40

Vorlaufzeit

Lieferung in 3-5 Werktagen

Kontakt

Telefon

+1 (314) 370-6046

orders@antibodies.com

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Produktdetails

Produktname

Anti-ROR gamma Antibody

Beschreibung

Goat polyclonal antibody to ROR gamma.

Anwendungen

ELISA, WB, IF, Flow Cytometry

Verdünnungen

ELISA: 1:16,000, WB: 0.01-0.1µg/ml, IF: 10µg/ml, Flow Cytometry: 10µg/ml

Reaktivität

Human

Immunogen

Synthetic peptide corresponding to the internal region, amino acids 200-212, of human ROR gamma.

Sequenz

CHLEYSPERGKAE

Wirt

Goat

Klonalität

Polyclonal

Isotyp

IgG

Konjugat

Unconjugated

Reinigung

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Konzentration

500 µg/ml

Molekulargewicht

55-60 kDa

Geschätztes MW

55.8kDa

Produktform

Liquid

Formulierung

Supplied in Tris Buffered Saline, pH 7.3, with 0.5% BSA and 0.02% Sodium Azide.

Lagerung

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze/thaw cycles.

Synonyme

IMD42, MGC129539, NR1F3, Nuclear receptor ROR gamma, Nuclear receptor ROR-gamma, Nuclear receptor RZR gamma, Nuclear receptor RZR-gamma, Nuclear receptor subfamily 1 group F member 3, RAR related orphan nuclear receptor variant 2, RAR related orphan receptor C, RAR related orphan receptor C, isoform a, RAR related orphan receptor gamma, RAR-related orphan receptor C, Retinoic acid binding receptor gamma, Retinoid related orphan receptor gamma, Retinoid-related orphan receptor-gamma, Rorc, RORG, RORG_HUMAN, RZR GAMMA, RZRG, TOR

Isotyp-Kontrollantikörper

Goat IgG (A121671)

Kompatible Sekundärantikörper

Haftungsausschluss

Dieses Produkt ist nur für Forschungszwecke bestimmt. Es ist nicht für diagnostische oder therapeutische Zwecke bestimmt.

Produktbilder (3)

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Figure 1: Western Blot - Anti-ROR gamma Antibody (A82850)

ROR gamma expression in Human Colon (A), Liver (B), and Testes (C) lysate analyzed by western blot. Cells were lysed in RIPA buffer and 35µg protein was run per lane. Primary incubation was performed with Anti-ROR gamma Antibody (A82850) at 0.1µg/ml (A), 0.03µg/ml (B), or 0.01µg/ml (C) and detected by chemiluminescence.

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Figure 2: Immunofluorescence - Anti-ROR gamma Antibody (A82850)

ROR gamma expression in U2OS cells analyzed by immunofluorescence. Cells were permeabilized with 0.15% Triton. Staining was performed with Anti-ROR gamma Antibody (A82850) at 10µg/ml for 1 hour and Alexa Fluor 488 secondary antibody at 2µg/ml. Cytoplasmic, plasma membrane, and nuclear staining shown and nuclei were stained with DAPI (blue). Negative control: Goat IgG Isotype Control at 10µg/ml followed by Alexa Fluor 488 secondary antibody at 2µg/ml.

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Figure 3: Flow Cytometry - Anti-ROR gamma Antibody (A82850)

ROR gamma expression in HeLa cells (blue line) analyzed by flow cytometry. Cells were fixed in PFA and permeabilized with 0.5% Triton. Staining was performed with Anti-ROR gamma Antibody (A82850) at 10µg/ml for 1 hour and Alexa Fluor 488 secondary antibody at 1µg/ml. Negative Control: Goat IgG Isotype Control (black line) followed by Alexa Fluor 488 secondary antibody.