Anti-ROCK1 Antikörper (A12783)

Name: Anti-ROCK1 Antibody
Brand: Antibodies.com
SKU: A12783
Price: 470.0 USD
Availability: InStock

Bild Vergrößern

$470

Polyklonal Kaninchen Antikörper gegen ROCK1 für WB und IHC.

100% Qualitätsgarantie

Preis-Match-Garantie

Versand

Versandkosten

$40

Vorlaufzeit

Lieferung in 5-8 Werktagen

Kontakt

Telefon

+1 (314) 370-6046

orders@antibodies.com

Garantien

100% Qualitätsgarantie

Preis-Match-Garantie

Stichworte:

Knockout-validierter Antikörper

Produktdetails

Produktname

Anti-ROCK1 Antibody

Beschreibung

Rabbit polyclonal antibody to ROCK1.

Anwendungen

WB, IHC

Verdünnungen

WB: 1:500-1:1,000, IHC: 1:50-1:100

Reaktivität

Human, Mouse, Rat

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 400-660 of human ROCK1 (NP_005397.1).

Sequenz

YSNRRYLSSANPNDNRTSSNADKSLQESLQKTIYKLEEQLHNEMQLKDEMEQKCRTSNIKLDKIMKELDEEGNQRRNLESTVSQIEKEKMLLQHRINEYQRKAEQENEKRRNVENEVSTLKDQLEDLKKVSQNSQLANEKLSQLQKQLEEANDLLRTESDTAVRLRKSHTEMSKSISQLESLNRELQERNRILENSKSQTDKDYYQLQAILEAERRDRGHDSEMIGDLQARITSLQEEVKHLKHNLEKVEGERKEAQDMLN

Wirt

Rabbit

Klonalität

Polyclonal

Isotyp

IgG

Konjugat

Unconjugated

Reinigung

Affinity purification.

Molekulargewicht

158 kDa

Produktform

Liquid

Formulierung

Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol and 0.02% Sodium Azide.

Lagerung

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Synonyme

coiled-coil-containing protein kinase 1, coiled-coil-containing protein kinase I, MGC131603, MGC43611, p160 Rhoassociated coiled coil-forming protein kinase, p160 ROCK-1, p160 ROCK1, p160ROCK, PRO0435, Renal carcinoma antigen NY REN 35, Renal carcinoma antigen NY-REN-35, Rho associated coiled coil containing protein kinase 1, Rho associated protein kinase 1, Rho kinase, Rho-alpha kinase, Rho-associated, Rho-associated protein kinase 1, ROCK I, ROCK-I, ROCK1_HUMAN, Rok, rokalpha

Isotyp-Kontrollantikörper

Kompatible Sekundärantikörper

Haftungsausschluss

Dieses Produkt ist nur für Forschungszwecke bestimmt. Es ist nicht für diagnostische oder therapeutische Zwecke bestimmt.

Produktbilder (4)

Bild Vergrößern

Figure 1: Western Blot - Anti-ROCK1 Antibody (A12783)

Western blot analysis of various lysates, using Anti-ROCK1 Antibody (A12783) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 10s.

Bild Vergrößern

Figure 2: Western Blot - Anti-ROCK1 Antibody (A12783)

Western blot analysis of C6, using Anti-ROCK1 Antibody (A12783) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 30s.

Bild Vergrößern

Figure 3: Western Blot - Anti-ROCK1 Antibody (A12783)

Western blot analysis of extracts from wild type(WT) and ROCK1 knockout (KO) 293T cells, using Anti-ROCK1 Antibody (A12783) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 10s.

Bild Vergrößern

Figure 4: Immunohistochemistry - Anti-ROCK1 Antibody (A12783)

Immunohistochemistry analysis of paraffin-embedded mouse spleen using Anti-ROCK1 Antibody (A12783) at a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.