Synthetic peptide within amino acids 1-50 of human RNF20 (NP_062538. 5).
Host
Rabbit
Clonality
Polyclonal
Isotype
IgG
Conjugate
Unconjugated
Purification
Antigen affinity purification.
Concentration
1 mg/ml
Product Form
Liquid
Formulation
Supplied in Tris-Citrate/Phosphate Buffer, pH 7-8, with 0.09% Sodium Azide.
Storage
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze/thaw cycles.
General Notes
This antibody was affinity purified using the immunising peptide immobilized on solid support. Immunoglobulin concentration was determined by extinction coefficient: absorbance at 280 nm of 1. 4 equals 1.0 mg of IgG.
Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Anti-RNF20 Antibody (A295761) was used for IP at 6 µg per reaction. RNF20 was also immunoprecipitated by a previous lot of this antibody and another rabbit anti-RNF20 antibody. For blotting immunoprecipitated RNF20, Anti-RNF20 Antibody (A295761) was used at 1 µg/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.
Samples: Whole cell lysate (50 µg) from HeLa, HEK293T, and mouse NIH 3T3 cells prepared using NETN lysis buffer. Antibody: Anti-RNF20 Antibody (A295761) was used for WB at 0.1 µg/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.
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