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Anti-RNA polymerase II CTD repeat YSPTSPS (phospho Thr4) Antibody (A309702)

Western Blot - Anti-RNA polymerase II CTD repeat YSPTSPS (phospho Thr4) Antibody (A309702) - Antibodies.com Western Blot - Anti-RNA polymerase II CTD repeat YSPTSPS (phospho Thr4) Antibody (A309702) - Antibodies.com Chromatin Immunoprecipitation - Anti-RNA polymerase II CTD repeat YSPTSPS (phospho Thr4) Antibody (A309702) - Antibodies.com
$450
Product Datasheet

Rabbit polyclonal antibody to RNA polymerase II CTD repeat YSPTSPS (phospho Thr4) for WB, IP and ChIP.

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Shipping Information

$40
Dispatched from St. Louis, MO.
Lead Time: 5-8 business days.

  • Specifications

Name
Anti-RNA polymerase II CTD repeat YSPTSPS (phospho Thr4) Antibody
Description
Rabbit polyclonal antibody to RNA polymerase II CTD repeat YSPTSPS (phospho Thr4).
Applications
WB, IP, ChIP
Dilutions
WB: 1:500-1:1,000, IP: 1:500-1:1,000, ChIP: 1:50-1:200
Reactivity
Human, Mouse, Rat
Immunogen
A phospho specific peptide corresponding to residues surrounding T4 of human POLR2A CTD repeat YSPTSPS.
Host
Rabbit
Clonality
Polyclonal
Isotype
IgG
Conjugate

Unconjugated

Purification
Affinity purification.
Molecular Weight
250 kDa
Product Form
Liquid
Formulation
Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol and 0.05% Proclin 300.
Storage
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Synonyms
3'-5' exoribonuclease, DNA-directed RNA polymerase II subunit A, DNA-directed RNA polymerase II subunit RPB1, DNA-directed RNA polymerase III largest subunit, EC:2.7.7.48, POLR2, RNA polymerase II subunit B1, RNA-directed RNA polymerase II subunit RPB1
Isotype Controls
Suitable Secondaries
See all Anti-Rabbit IgG Secondaries →
Disclaimer
This product is for research use only. It is not intended for diagnostic or therapeutic use.

  • Scientific Validation DataValidation Data (3)

Western Blot - Anti-RNA polymerase II CTD repeat YSPTSPS (phospho Thr4) Antibody (A309702) - Antibodies.comEnlarge Image

Western Blot - Anti-RNA polymerase II CTD repeat YSPTSPS (phospho Thr4) Antibody (A309702)

Western blot analysis of extracts of various cell lines, using Anti-RNA polymerase II CTD repeat YSPTSPS (phospho Thr4) Antibody (A309702) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 90s.
Western Blot - Anti-RNA polymerase II CTD repeat YSPTSPS (phospho Thr4) Antibody (A309702) - Antibodies.comEnlarge Image

Western Blot - Anti-RNA polymerase II CTD repeat YSPTSPS (phospho Thr4) Antibody (A309702)

Immunoprecipitation analysis of 300µg extracts of C6 cells using 3µg of Anti-RNA polymerase II CTD repeat YSPTSPS (phospho Thr4) Antibody (A309702). This Western blot was performed on the immunoprecipitate using Anti-RNA polymerase II CTD repeat YSPTSPS (phospho Thr4) Antibody (A309702) at a dilution of 1:1000.
Chromatin Immunoprecipitation - Anti-RNA polymerase II CTD repeat YSPTSPS (phospho Thr4) Antibody (A309702) - Antibodies.comEnlarge Image

Chromatin Immunoprecipitation - Anti-RNA polymerase II CTD repeat YSPTSPS (phospho Thr4) Antibody (A309702)

Chromatin immunoprecipitation (ChIP) analysis of extracts of 293T; cells, using Anti-RNA polymerase II CTD repeat YSPTSPS (phospho Thr4) Antibody (A309702) and Rabbit IgG. The amount of immunoprecipitated DNA was checked by quantitative PCR. Histogram was constructed by the ratios of the immunoprecipitated DNA to the input.
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