Anti-Rhodopsin Antikörper [4D2] (A305179)

Name: Anti-Rhodopsin Antibody [4D2]
Brand: Antibodies.com
SKU: A305179
Price: 500.0 USD
Availability: InStock

Dieses Produkt entspricht Sigma (mabn15). Entdecken Sie mehr für weniger.

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$500

Monoklonal [4D2] Maus Antikörper gegen Rhodopsin für WB, IHC, ICC/IF, IP und ELISA.

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Versand

Versandkosten

$40

Vorlaufzeit

Lieferung in 5-8 Werktagen

Kontakt

Telefon

+1 (314) 370-6046

orders@antibodies.com

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Produktdetails

Produktname

Anti-Rhodopsin Antibody [4D2]

Beschreibung

Mouse monoclonal [4D2] antibody to Rhodopsin.

Spezifität

This antibody binds specifically to the N-terminus of Rhodopsin. It does not detect Rhodopsin in invertebrates.

Anwendungen

WB, IHC, ICC/IF, IP, ELISA

Verdünnungen

WB: 1:1,000, IHC: 1,000

Reaktivität

Mammalian, Fish, Avian, Amphibians, Shark

Immunogen

Bovine Rhodopsin.

Wirt

Mouse

Klonalität

Monoclonal

Klon

4D2

Isotyp

IgG1

Konjugat

Unconjugated

Reinigung

Protein G purification.

Konzentration

1 mg/ml

Molekulargewicht

~40 kDa

Produktform

Liquid

Formulierung

Supplied in Phosphate Buffered Saline, pH 7.4, with 50% Glycerol and 0.09% Sodium Azide.

Lagerung

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Synonyme

CSNBAD1, MGC138309, MGC138311, OPN 2, OPN2, opsd, OPSD_HUMAN, opsin 2, Opsin 2 rod pigment, Opsin-2, Opsin2, Retinitis Pigmentosa 4, Retinitis pigmentosa 4 autosomal dominant, RHO, RP 4, RP4

Isotyp-Kontrollantikörper

Kompatible Sekundärantikörper

Haftungsausschluss

Dieses Produkt ist nur für Forschungszwecke bestimmt. Es ist nicht für diagnostische oder therapeutische Zwecke bestimmt.

Produktbilder (3)

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Figure 1: Western Blot - Anti-Rhodopsin Antibody [4D2] (A305179)

Western blot analysis of Bovine photoreceptor membranes showing detection of Rhodopsin protein using Anti-Rhodopsin Antibody [4D2] (A305179) at 1:1,000. Lane 1: MW ladder. Lane 2: 10 ug. Lane 3: 5 ug. Lane 4: 2.5 ug..

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Figure 2: Western Blot - Anti-Rhodopsin Antibody [4D2] (A305179)

Western blot analysis of human A549 cells showing detection of ~38.9kDa Rhodopsin protein using Anti-Rhodopsin Antibody [4D2] (A305179) at 1:1,000 for 2. Lane 1: MW ladder. Lane 2: human A549 Cells 15 ug). Load: 15 ug. Block: 5% Skim Milk Powder in TBST. 5 hours at room temperature with shaking. The secondary antibody used was Goat anti-mouse IgG:HRP at 1:1,000 for 1 hour at room temperature with shaking. Color Development: Chemiluminescent for HRP (Moss) for 5 minutes in room temperature. Predicted/Observed Size: ~38.9kDa. Other Band(s): Band appears at ~75 kDa indicating detection of the Rhodopsin dimer.

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Figure 3: Immunohistochemistry - Anti-Rhodopsin Antibody [4D2] (A305179)

Immunohistochemistry analysis of mouse retina. The Primary Antibody used was Anti-Rhodopsin Antibody [4D2] (A305179) at 1:1,000. The secondary antibody used was FITC Goat Anti-Mouse (green). Counterstain: DAPI (blue) nuclear stain. Localization: Staining of photoreceptor outer segment (OS). Other layers of the retina: IS inner segment; ONL outer nuclear layer; OPL outer plexiform layer; INL inner nuclear layer; IPL inner plexiform layer; GCL ganglion cell layer..