This antibody recognises the sequence TETSQVAPA corresponding to aa 340 to 348 of bovine rhodopsin, a pigment of the retina responsible for the formation of photoreceptor cells and the first events in the perception of light. Rhodopsin consists of the protein opsin and a reversibly bound cofactor, retinal, a photoreactive chromophore. Upon light absorption the 11-cis-retinal is isomerised to all-trans retinal, leading to a change in the shape of rhodopsin. The change activates the associated G protein and triggers a second messenger cascade, eventually resulting in the transmission of a nerve impulse to the brain. The trans-retinal is released and converted back into 11-cis-retinal for re-entry into the cycle.Defects in the gene cause various retinopathies such as retinitis pigmentosa and X-linked congenital stationary night blindness.
Anwendungen
WB, IHC-P, IP, IF
Verdünnungen
IHC-P: 1:100, WB: 1:1,000
Reaktivität
Bovine, Mammals, Amphibia
Immunogen
Bovine rhodopsin.
Wirt
Mouse
Klonalität
Monoclonal
Klon
1D4
Isotyp
IgG1
Konjugat
Unconjugated
Reinigung
Protein G affinity chromatography of tissue culture supernatant.
Konzentration
Lot Specific
Produktform
Liquid
Formulierung
Supplied in 10mM HEPES, pH 7.5, with 150mM NaCl, 0.01% BSA, 50% Glycerol, and 0.05% Sodium Azide.
Lagerung
Shipped at ambient temperature. Upon delivery aliquot and store at -20°C. When thawed, aliquot the sample as needed. Short term (up to 4 weeks): store at 4°C. Long term: store at -20°C. Avoid freeze / thaw cycles. Storage in frost free freezers is not recommended.
Allgemeine Hinweise
Mouse anti Rhodopsin antibody, clone 1D4 recognizes the sequence TETSQVAPA corresponding to aa 340 to 348 of bovine rhodopsin, a pigment of the retina responsible for the formation of photoreceptor cells and the first events in the perception of light. Rhodopsin consists of the protein opsin and a reversibly bound cofactor, retinal, a photoreactive chromophore. Upon light absorption the 11-cis-retinal is isomerised to all-trans retinal, leading to a change in the shape of rhodopsin. The change activates the associated G protein and triggers a second messenger cascade, eventually resulting in the transmission of a nerve impulse to the brain. The trans-retinal is released and converted back into 11-cis-retinal for re-entry into the cycle.Defects in the gene cause various retinopathies such as retinitis pigmentosa and X-linked congenital stationary night blindness.