Anti-RhoA Antibody (A12591)

Name: Anti-RhoA Antibody
Brand: Antibodies.com
SKU: A12591
Price: 355.0 USD
Availability: InStock

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$355

Rabbit polyclonal antibody to RhoA for WB, IHC and ICC/IF.

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Shipping Information

Freight/Packing Charges: $40

Dispatched from St. Louis, MO.

Lead Time: 7-10 business days.

Specifications

Name

Anti-RhoA Antibody

Description

Rabbit polyclonal antibody to RhoA.

Applications

WB, IHC, ICC/IF

Dilutions

WB: 1:500-1:1,000, IHC: 1:50-1:200, ICC/IF: 1:50-1:200

Reactivity

Human, Mouse, Rat

Immunogen

A synthetic peptide corresponding to a sequence within amino acids 94-193 of human RhoA (NP_001655.1).

Sequence

NIPEKWTPEVKHFCPNVPIILVGNKKDLRNDEHTRRELAKMKQEPVKPEEGRDMANRIGAFGYMECSAKTKDGVREVFEMATRAALQARRGKKKSGCLVL

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Conjugate

Unconjugated

Purification

Affinity purification.

Concentration

Lot Specific

Molecular Weight

21 kDa

Product Form

Liquid

Formulation

Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol and 0.01% Thiomersal.

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze/thaw cycles.

Synonyms

ARH12, ARHA, h12, Rho cDNA clone 12, RHO12, Transforming protein RhoA

Isotype Controls

Suitable Secondaries

See all Anti-Rabbit IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation DataValidation Data (3)

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Western Blot - Anti-RhoA Antibody (A12591)

Western blot analysis of extracts of various cell lines, using Anti-RhoA Antibody (A12591) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 90s.

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Immunohistochemistry - Anti-RhoA Antibody (A12591)

Immunohistochemistry analysis of paraffin-embedded human placenta using Anti-RhoA Antibody (A12591) at a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

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Immunofluorescence - Anti-RhoA Antibody (A12591)

Immunofluorescence analysis of NIH-3T3 cells using Anti-RhoA Antibody (A12591) at a dilution of 1:100 (40x lens). DAPI was used to stain the cell nuclei (blue).

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