Anti-Retinol Binding Protein 4 Aptamer [RBA] (Biotin) (A320915)

Anti-Retinol Binding Protein 4 Aptamer [RBA] (Biotin)

Synthetic DNA aptamer to Retinol Binding Protein 4 [RBA] (Biotin).

No significant signal was observed from Vaspin, Nampt, adiponectin, human serum albumin, human IgG, and fibrinogen.

Biotin

Resuspend to 100µM with 10mM Tris-HCl, pH 7.5, and 0.1mM EDTA.

Binding Buffer: 25mM Tris-HCl Buffer, pH 7.6, with 100mM Sodium Chloride, 2mM Magnesium Chloride, 5mM Potassium Chloride, 1mM Calcium Chloride, and 0.02% Tween 20. Binding Temperature: 25°C.

5'-dApdTpdApdCpdCpdApdGpdCpdTpdTpdApdTpdTpdCpdApdApdTpdTpdApdCpdApdGpdTpdApdGpdTpdGpdApdGpdGpdGpdGpdTpdCpdCpdGpdTpdCpdGpdTpdGpdGpdGpdGpdTpdApdGpdTpdTpdGpdGpdGpdTpdCpdGpdTpdGpdGpdApdGpdApdTpdApdGpdTpdApdApdGpdTpdGpdCpdApdApdTpdCpdTp-3'

76 nt

Affinity (Kd) = 201 nM

755800 M^-1cm^-1

HPLC

Lyophilized

Reconstitution dependent.

Lyophilized from nuclease-free water (no residual salts).

Shipped at 4°C. Lyophilized: Store at -20°C. Reconstituted: Store short term at 4°C (i.e. use within days) or aliquot and store at -20°C (medium term: i.e. use within weeks) or -80°C long term. Avoid freeze/thaw cycles.

Aptamers have not been validated by Antibodies.com and they need to be tested/evaluated under identical conditions to which they were reported in the literature including, but not limited to, temperature and matrix/buffer conditions. All aptamers are manufactured on request and have a lead time of 30-45 business days.