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Rekombinant Phl p 7 (7.0101) Protein (A242916)

Flow Cytometry - Recombinant Phl p 7 (7.0101) Protein (A242915) - Antibodies.com SDS-PAGE - Recombinant Phl p 7 (7.0101) Protein (A242916) - Antibodies.com ELISA - Recombinant Phl p 7 (7.0101) Protein (A242916) - Antibodies.com
$525
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$40
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Lieferung in 4-6 Werktagen

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Produktname
Recombinant Phl p 7 (7.0101) Protein
Anwendungen
ELISA, Flow Cytometry, WB
Ausdruckssystem
Escherichia coli
Natur
Recombinant
Spezies
Phleum pratense (Timothy Grass)
Sequenz
DNA sequence encoding 90 amino acids was fused with a Strep Tag at the N-terminus to form this recombinant protein.
Etikett
Strep Tag (N-terminus)
Voraussichtliches MW
10 kDa
Konjugat

Unconjugated

Reinigung
Ion exchange and affinity chromatography.
Produktform
Lyophilized
Konzentration
Reconstitution dependent.
Formulierung
Lyophilized from 100mM Tris Buffer, pH 8, with 150mM NaCl, and without preservatives or carriers (0.2 µm filter sterilized).
Lagerung
Shipped at 4°C. Lyophilized: Store at -20°C to -80°C. Reconstituted: Use immediately or aliquot and store at -20°C to -80°C. Avoid freeze/thaw cycles.
Haftungsausschluss
Dieses Produkt ist nur für Forschungszwecke bestimmt. Es ist nicht für diagnostische oder therapeutische Zwecke bestimmt.
Flow Cytometry - Recombinant Phl p 7 (7.0101) Protein (A242915) - Antibodies.comBild Vergrößern
Figure 1: Flow Cytometry - Recombinant Phl p 7 (7.0101) Protein (A242916)
Flow cytometry dot-plot staining pattern of Recombinant Phl p 7 (7.0101) Protein stimulated human peripheral whole blood lymphocytes and basophils of a proven allergic donor stained using Anti-CD63 [MEM-259] (FITC) Antibody and Anti-CD203c [NP4D6] (PE) Antibody.
SDS-PAGE - Recombinant Phl p 7 (7.0101) Protein (A242916) - Antibodies.comBild Vergrößern
Figure 2: SDS-PAGE - Recombinant Phl p 7 (7.0101) Protein (A242916)
Purity verification: 5 µg of Recombinant Phl p 7 (7.0101) Protein with > 95 % purity checked by Coomassie Brilliant Blue stained SDS-PAGE.
ELISA - Recombinant Phl p 7 (7.0101) Protein (A242916) - Antibodies.comBild Vergrößern
Figure 3: Recombinant Phl p 7 (7.0101) Protein (A242916)
An ELISA test was designed to prove the bond between the coated target, Recombinant Phl p 7 (7.0101) Protein, and allergen-specific human plasma IgG4 antibodies of Phleum pratense positive donor. A measurable signal was subsequently generated by the addition of Anti-Human IgG4 Antibody (Biotin), Streptavidin-HRP, and substrate solution (TMB). The intensity of the signal is proportional to the amount of coated Recombinant Phl p 7 (7.0101) Protein.
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