Rekombinant Human TNF beta Protein (N-terminal His Tag) (A61474)

Recombinant Human TNF beta Protein (N-terminal His Tag)

Cytotoxic Assay

Escherichia coli

Recombinant

Human

Protein fragment

MGSSHHHHHH SSGLVPRGSH MLPGVGLTPS AAQTARQHPK MHLAHSTLKP AAHLIGDPSK QNSLLWRANT DRAFLQDGFS LSNNSLLVPT SGIYFVYSQV VFSGKAYSPK ATSSPLYLAH EVQLFSSQYP FHVPLLSSQK MVYPGLQEPW LHSMYHGAAF QLTQGDQLST HTDGIPHLVL SPSTVFFGAF AL

N-terminal His Tag

20.9 kDa

ED50: <0.3 ng/ml, as determined in a cytotoxicity assay using L929 mouse fibrosarcoma cells in the presence of the metabolic inhibitor actinomycin D.

>90% as determined by SDS-PAGE.

Liquid

0.25mg/ml

Supplied in 20 mM Tris HCl Buffer, pH 8, with 100 mM Sodium Chloride, 1 mM DTT, and 30% Glycerol.

Shipped at 4°C. Short term (2-4 weeks) store at 4°C. Long term store at -20°C, it is recommended to add a carrier protein (0.1% HSA or BSA). Avoid freeze/thaw cycles.

DIF, LT, LT alpha, LT-alpha, Lta, LTalpha, Lymphotoxin alpha, Lymphotoxin alpha (TNF superfamily, member 1), Lymphotoxin-alpha, TNF B, TNF superfamily member 1, TNF, lymphocyte-derived, TNF-beta, TNFB, TNFbeta, TNFB_HUMAN, TNFSF1, TNLG1E, Tumor necrosis factor beta, tumor necrosis factor ligand 1E, Tumor necrosis factor ligand superfamily member 1