Recombinant Human Lysozyme is added in lysing bacterial cells to the cell extraction buffer at a range of 0.05-0.5mg/ml (7,000-70,000 U/ml). The common extraction buffer for E. coli cells contains 0.2mg/ml of recombinant Lysozyme in 100mM Tris-HCl, 2mM EDTA, 0.05% Triton X-100, pH 8. The cell paste is suspended in extraction buffer and incubated for at least 15 min at room temperature. Alternatively, Triton X-100 at 1% and PMSF at 1mM can be added separately and the lysis solution is further incubated for at least 15 min at 37C. In other cases, freeze-thaw cycles, sonication, and mechanical cell disruptions are also used to complement the lysozyme lysis protocol.
Ausdruckssystem
Rice
Natur
Recombinant
Spezies
Human
Proteinlänge
Protein fragment
Voraussichtliches MW
14 kDa
Biologisch Aktiv
Yes
Biologische Aktivität
>100,000 U/mg protein.
Einheitendefinition
One unit will produce a change of A450 0.001 per minute at pH 6.24 and 25°C, using a suspension of micrococcus lysodeikticus as substrate in a 2.6ml reaction volume.
Reinheit
>85% as determined by SDS-PAGE.
Produktform
Lyophilized
Konzentration
1mg/ml
Formulierung
Lyophilized without additives.
Lagerung
Shipped at ambient temperature. Lyophilized: Short term (3 weeks) store at 25°C. Long term store desiccated below -18°C. Reconstituted: Short term (2-7 days) store at 4°C. Long term store below -18°C, it is recommended to add a carrier protein (0.1% HSA or BSA). Avoid freeze/thaw cycles.
