Recombinant Human ICOS Ligand/ICOSL Protein (Fc Chimera 6xHis Tag)
Anwendungen
SDS-PAGE, Flow Cytometry
Ausdruckssystem
HEK293 cells
Natur
Recombinant
Spezies
Human
Proteinlänge
Protein fragment
Sequenz
B7H2 (Asp19-Thr256) + mFc (Pro99-Lys330) + 6xHis Tag
Etikett
C-terminal Mouse Fc Tag and 6xHis Tag
Molekulargewicht
The protein has a predicted molecular mass of 53.9 kDa after removal of the signal peptide.
Konjugat
Unconjugated
Reinheit
> 95%, by SDS-PAGE and Coomassie blue staining.
Produktform
Lyophilized
Konzentration
Reconstitution dependent.
Formulierung
Lyophilized from sterile Phosphate Buffered Saline, pH 7.4. Normally 5%–8% Trehalose is added as a protectant before lyophilization.
Lagerung
Shipped at 4°C. Lyophilized: Store at -20°C to -80°C. Reconstituted: Aliquot and store at -80°C. Product is stable for one year. Avoid freeze/thaw cycles.
Haftungsausschluss
Dieses Produkt ist nur für Forschungszwecke bestimmt. Es ist nicht für diagnostische oder therapeutische Zwecke bestimmt.
Figure 2: Flow Cytometry - Recombinant Human ICOS Ligand/ICOSL Protein (Fc Chimera 6xHis Tag) (A318379)
HEK293 cell line was transfected with: Irrelevant protein (B) and human ICOS (A). Cells were surface stained with 1µg/ml of Recombinant Human ICOS Ligand/ICOSL Protein (Fc Chimera 6xHis Tag) (A318379) followed by Anti-Mouse IgG Antibody (Alexa 488).
Figure 3: Recombinant Human ICOS Ligand/ICOSL Protein (Fc Chimera 6xHis Tag) (A318379)
ELISA plates were pre-coated with Recombinant Human ICOS Ligand/ICOSL Protein (Fc Chimera 6xHis Tag) (A318379) at 1 µg/ml (100 µl/well) which can bind Anti-B7-H2 Rabbit mAb in a linear range of 3.2-80 ng/ml.
