Recombinant Human CTLA4 Protein (Fc Chimera 6xHis Tag)
Applications
ELISA, SDS-PAGE
Expression System
HEK293 cells
Nature
Recombinant
Protein Species
Human
Protein Length
Protein fragment
Sequence
CTLA-4 (Gln36-Ser161) + mFc (Pro99-Lys330) + 6xHis Tag
Tag
C-terminal Mouse Fc Tag and 6xHis Tag
Molecular Weight
The protein has a predicted molecular mass of 54 kDa after removal of the signal peptide.
Conjugate
Unconjugated
Purity
> 95%, by SDS-PAGE and Coomassie blue staining.
Product Form
Lyophilized
Concentration
Reconstitution dependent.
Formulation
Lyophilized from sterile Phosphate Buffered Saline, pH 7.4. Normally 5%–8% Trehalose is added as a protectant before lyophilization.
Storage
Shipped at 4°C. Lyophilized: Store at -20°C to -80°C. Reconstituted: Aliquot and store at -80°C. Product is stable for one year. Avoid freeze/thaw cycles.
Synonyms
CD152, Cytotoxic T-lymphocyte protein 4, Cytotoxic T-lymphocyte-associated antigen 4
Disclaimer
This product is for research use only. It is not intended for diagnostic or therapeutic use.
Recombinant Human CTLA4 Protein (Fc Chimera 6xHis Tag) (A318391)
ELISA plates were pre-coated with Recombinant Human CD80 Protein (Fc Tag) (A318255) at 2 µg/ml (100 µl/well) which can bind Recombinant Human CTLA4 Protein (Fc Chimera 6xHis Tag) (A318391) in a linear range of 0.048-2.094 ng/ml.
Recombinant Human CTLA4 Protein (Fc Chimera 6xHis Tag) (A318391)
ELISA plates were pre-coated with Recombinant Human CD86 Protein (Fc Tag) (A318254) at 2 µg/ml (100 µl/well) which can bind Recombinant Human CTLA4 Protein (Fc Chimera 6xHis Tag) (A318391) in a linear range of 0.048-2.694 ng/ml.
Recombinant Human CTLA4 Protein (Fc Chimera 6xHis Tag) (A318391)
ELISA plates were pre-coated with Recombinant Human CTLA4 Protein (Fc Chimera 6xHis Tag) (A318391) at 2 µg/ml (100 µl/well) which can bind Ipilimumab Biosimilar - Anti-CTLA4 Antibody - BSA and Azide free (A318934) in a linear range of 0.13-16.0 ng/ml.
Flow Cytometry - Recombinant Human CTLA4 Protein (Fc Chimera 6xHis Tag) (A318391)
HEK293 cell line was transfected with: Irrelevant protein (B) and human B7-2 (A). Cells were surface stained with 1µg/ml of Recombinant Human CTLA4 Protein (Fc Chimera 6xHis Tag) (A318391) followed by Anti-Mouse IgG Antibody (Alexa 488).
Flow Cytometry - Recombinant Human CTLA4 Protein (Fc Chimera 6xHis Tag) (A318391)
Flow cytometry analysis of 1µg/ml of Recombinant Human CTLA4 Protein (Fc Chimera 6xHis Tag) (A318391) on Expi293 cells transfected with human B7-1 (Blue histogram) or Expi293 transfected with irrelevant protein (Red histogram).
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