The protein has a predicted molecular mass of 51.5 kDa after removal of the signal peptide.
Conjugate
Unconjugated
Purity
> 95%, by SDS-PAGE and Coomassie blue staining.
Product Form
Lyophilized
Concentration
Reconstitution dependent.
Formulation
Lyophilized from sterile Phosphate Buffered Saline, pH 7.4. Normally 5%–8% Trehalose is added as a protectant before lyophilization.
Storage
Shipped at 4°C. Lyophilized: Store at -20°C to -80°C. Reconstituted: Aliquot and store at -80°C. Product is stable for one year. Avoid freeze/thaw cycles.
ELISA plates were pre-coated with Recombinant Human CD86 Protein (Fc Tag) (A318254) at 2 µg/ml (100 µl/well) which can bind Recombinant Human CTLA4 Protein (Fc Chimera 6xHis Tag) (A318391) in a linear range of 0.048-2.694 ng/ml.
ELISA plates were pre-coated with Recombinant Human CD86 Protein (Fc Tag) (A318254) at 2 µg/ml (100 µl/well) which can bind Recombinant Human CD28 Protein (Fc Chimera 6xHis Tag) (A318394) in a linear range of 62.5-4000 ng/ml.
Flow Cytometry - Recombinant Human CD86 Protein (Fc Tag) (A318254)
HEK293 cell line was transfected with: Irrelevant protein (B) and human CD28 (A). Cells were surface stained with 1µg/ml of Recombinant Human CD86 Protein (Fc Tag) (A318254) followed by Anti-Human IgG Antibody (Alexa 488).
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