Recombinant Human CD80 Protein (Fc Chimera 6xHis Tag)
Applications
ELISA, SDS-PAGE
Expression System
HEK293 cells
Nature
Recombinant
Protein Species
Human
Protein Length
Protein fragment
Sequence
B7-1 (Val35-Asn242) + mFc (Pro99-Lys330) + 6xHis Tag
Tag
C-terminal Mouse Fc Tag and 6xHis Tag
Molecular Weight
The protein has a predicted molecular mass of 50.9 kDa after removal of the signal peptide. The apparent molecular mass of B7-1-mFc-His is approximately 70-130 kDa due to glycosylation.
Conjugate
Unconjugated
Purity
> 95%, by SDS-PAGE and Coomassie blue staining.
Product Form
Lyophilized
Concentration
Reconstitution dependent.
Formulation
Lyophilized from sterile Phosphate Buffered Saline, pH 7.4. Normally 5%–8% Trehalose is added as a protectant before lyophilization.
Storage
Shipped at 4°C. Lyophilized: Store at -20°C to -80°C. Reconstituted: Aliquot and store at -80°C. Product is stable for one year. Avoid freeze/thaw cycles.
Disclaimer
This product is for research use only. It is not intended for diagnostic or therapeutic use.
Recombinant Human CD80 Protein (Fc Chimera 6xHis Tag) (A318363)
ELISA plates were pre-coated with Recombinant Human CD80 Protein (Fc Chimera 6xHis Tag) (A318363) at 1 µg/ml (100 µl/well) which can bind anti-B7-1 monoclonal antibody,Rabbit mAb clone: DM111 in a linear range of 0.13-80 ng/ml.
Flow Cytometry - Recombinant Human CD80 Protein (Fc Chimera 6xHis Tag) (A318363)
Flow cytometry analysis of 15µg/ml of Recombinant Human CD80 Protein (Fc Chimera 6xHis Tag) (A318363) on Expi293 cells transfected with human CD28 (Blue histogram) or Expi293 transfected with irrelevant protein (Red histogram).
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