Recombinant Human CD28 Protein (Fc Chimera 6xHis Tag)
Anwendungen
ELISA, SDS-PAGE
Ausdruckssystem
HEK293 cells
Natur
Recombinant
Spezies
Human
Proteinlänge
Protein fragment
Sequenz
CD28 (Asn19-Pro152) + mFc (Pro99-Lys330) + 6xHis Tag
Etikett
C-terminal Mouse Fc Tag and 6xHis Tag
Molekulargewicht
The protein has a predicted molecular mass of 68-72 kDa after removal of the signal peptide.
Konjugat
Unconjugated
Reinheit
> 95%, by SDS-PAGE and Coomassie blue staining.
Produktform
Lyophilized
Konzentration
Reconstitution dependent.
Formulierung
Lyophilized from sterile Phosphate Buffered Saline, pH 7.4. Normally 5%–8% Trehalose is added as a protectant before lyophilization.
Lagerung
Shipped at 4°C. Lyophilized: Store at -20°C to -80°C. Reconstituted: Aliquot and store at -80°C. Product is stable for one year. Avoid freeze/thaw cycles.
Synonyme
CD 28, CD28 antigen, CD28 molecule, CD28_HUMAN, MGC138290, T cell antigen CD28, T cell specific surface glycoprotein, T cell specific surface glycoprotein CD28, T-cell-specific surface glycoprotein CD28, TP44
Haftungsausschluss
Dieses Produkt ist nur für Forschungszwecke bestimmt. Es ist nicht für diagnostische oder therapeutische Zwecke bestimmt.
Figure 2: Recombinant Human CD28 Protein (Fc Chimera 6xHis Tag) (A318394)
ELISA plates were pre-coated with Recombinant Human CD80 Protein (Fc Tag) (A318255) at 2 µg/ml (100 µl/well) which can bind Recombinant Human CD28 Protein (Fc Chimera 6xHis Tag) (A318394) in a linear range of 125-4000 ng/ml.
Figure 3: Recombinant Human CD28 Protein (Fc Chimera 6xHis Tag) (A318394)
ELISA plates were pre-coated with Recombinant Human CD86 Protein (Fc Tag) (A318254) at 2 µg/ml (100 µl/well) which can bind Recombinant Human CD28 Protein (Fc Chimera 6xHis Tag) (A318394) in a linear range of 62.5-4000 ng/ml.
Figure 4: Flow Cytometry - Recombinant Human CD28 Protein (Fc Chimera 6xHis Tag) (A318394)
HEK293 cell line was transfected with: Irrelevant protein (B) and human B7-2 (A). Cells were surface stained with 1µg/ml of Recombinant Human CD28 Protein (Fc Chimera 6xHis Tag) (A318394) followed by Anti-Mouse IgG Antibody (Alexa 488).
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