Recombinant Human CD137 Protein (Fc Chimera 6xHis Tag)
Applications
SDS-PAGE, Flow Cytometry
Expression System
HEK293 cells
Nature
Recombinant
Protein Species
Human
Protein Length
Protein fragment
Sequence
41BB (Leu24-Gln186) + mFc (Pro99-Lys330) + 6xHis Tag
Tag
C-terminal Mouse Fc Tag and 6xHis Tag
Molecular Weight
The protein has a predicted molecular mass of 54-60 kDa after removal of the signal peptide.
Conjugate
Unconjugated
Purity
> 95%, by SDS-PAGE and Coomassie blue staining.
Product Form
Lyophilized
Concentration
Reconstitution dependent.
Formulation
Lyophilized from sterile Phosphate Buffered Saline, pH 7.4. Normally 5%–8% Trehalose is added as a protectant before lyophilization.
Storage
Shipped at 4°C. Lyophilized: Store at -20°C to -80°C. Reconstituted: Aliquot and store at -80°C. Product is stable for one year. Avoid freeze/thaw cycles.
Flow Cytometry - Recombinant Human CD137 Protein (Fc Chimera 6xHis Tag) (A318397)
HEK293 cell line was transfected with: Irrelevant protein (B) and human 4-1BB Ligand (A). Cells were surface stained with 1µg/ml of Recombinant Human CD137 Protein (Fc Chimera 6xHis Tag) (A318397) followed by Anti-Mouse IgG Antibody (Alexa 488).
Recombinant Human CD137 Protein (Fc Chimera 6xHis Tag) (A318397)
ELISA plates were pre-coated with Recombinant Human CD137 Protein (Fc Chimera 6xHis Tag) (A318397) at 2 µg/ml (100 µl/well) which can bind Utomilumab Biosimilar - Anti-CD137 Antibody - BSA and Azide free (A318919) in a linear range of 3.2-400 ng/ml.
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