Rekombinant E. coli Glucokinase Protein (N-terminal His Tag) (A325813)

Recombinant E. coli Glucokinase Protein (N-terminal His Tag)

SDS-PAGE

Escherichia coli

Recombinant

E. coli

Full length protein

MGSSHHHHHH SSGLVPRGSH MGSMTKYALV GDVGGTNARL ALCDIASGEI SQAKTYSGLD YPSLEAVIRV YLEEHKVEVK DGCIAIACPI TGDWVAMTNH TWAFSIAEMK KNLGFSHLEI INDFTAVSMA IPMLKKEHLI QFGGAEPVEG KPIAVYGAGT GLGVAHLVHV DKRWVSLPGE GGHVDFAPNS EEEAIILEIL RAEIGHVSAE RVLSGPGLVN LYRAIVKADN RLPENLKPKD ITERALADSC TDCRRALSLF CVIMGRFGGN LALNLGTFGG VFIAGGIVPR FLEFFKASGF RAAFEDKGRF KEYVHDIPVY LIVHDNPGLL GSGAHLRQTL GHIL

N-terminal His Tag

37.1 kDa

>70 U/mg obtained by measuring the increase of NADPH in absorbance at 340 nm resulting from the reduction of NADP.

One unit is defined as the ammount of enzyme that will oxidize 1 µmole of Glucose to D-glucose 6-phosphate per minute in the presence of Beta-NADP at pH 9.0 at 37C.

>95% as determined by SDS-PAGE.

Liquid

1 mg/ml

Supplied in 20 mM Tris HCl Buffer, pH 8, with 150 mM Sodium Chloride and 10% Glycerol.

Shipped at 4°C. Short term (2-4 weeks) store at 4°C. Long term store at -20°C, it is recommended to add a carrier protein (0.1% HSA or BSA). Avoid freeze/thaw cycles.

ATP:D-hexose 6-phosphotransferase, FGQTL3, GCK, GK, GLK, Hexokinase D pancreatic isozyme, Hexokinase type IV, Hexokinase-4, Hexokinase-D, HHF3, HK IV, HK4, HKIV, HXK4_HUMAN, HXKP, LGLK, MODY2