Rat IgG total ELISA Kit is based on the quantitative sandwich enzyme-linked immunosorbent assay technique to measure concentration of rat IgG in the samples. A monoclonal antibody specific for rat IgG has been pre-coated onto a microplate. Standards and samples are pipetted into the wells for the first incubation. After washing away any unbound substances, a HRP-linked detect antibody specific for IgG is added to the wells. After washing, substrate solution is added to the wells and color develops in proportion to the amount of IgG bound in the initial step. The color development is stopped and the intensity of the color is measured.
Plattform
Microplate
Nachweismethode
Colorimetric
Probentyp
Cell culture supernatant, serum and plasma.
Sensitivität
0.20 ng/ml
Detektionsbereich
1.56 - 100 ng/ml
Reaktivität
Rat
Precision
Intra-assay CV: < 8%, Inter-assay CV: < 10%
Lagerung
Store at 2 - 8°C.
Synonyms
Ig gamma, Ig gamma 1 chain C region, Ig gamma 2 chain C region, Ig gamma 3 chain C region, Ig gamma 4 chain C region, Ig gamma-1 chain C region, IGHG1, IGHG2, IGHG3, IGHG4, Immunoglobin heavy constant gamma 1, Immunoglobulin G
Haftungsausschluss
Dieses Produkt ist nur für Forschungszwecke bestimmt. Es ist nicht für diagnostische oder therapeutische Zwecke bestimmt.